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Title: Effect of the exposure to fine inhalable particulate matter (pm2.5) on sperm functional quality of mice
Authors: Intasqui, Paula
Tamashiro, Letícia Kaory
Yariwake, Victor Yuji
Souza, Rosana Xavier
Kanashiro, Camila Matie
Veras, Mariana Matera
Keywords: Air pollution
DNA Fragmentation
Oxidative Stress
Issue Date: 1-Sep-2020
Publisher: American Society for Reproductive Medicine
Abstract: Objective: To evaluate the effect of exposure to pollution (fine inhalable particulate matter - PM2.5) from the city of São Paulo on sperm functional quality. Design: Male isogenic BALB/c mice were used, distributed in two groups, control (n=6) and polluted air (n=8). For the polluted air group, after weaning (21 days), animals were daily exposed to 600 μg/m3 of PM2,5 for 96 days in an Ambient Particle Concentrator (APC). Control group was simultaneously exposed to filtered air in the APC. On postnatal day 118, animals were sacrificed (isoflurane overdose), body was weighted and the epididymis were collected. Materials and Methods: Sperm obtained from the cauda epididymis were used for the evaluation of motility, mitochondrial activity (DAB staining), acrosome integrity (PNA staining), DNA fragmentation (alkaline comet assay), oxidative stress (DHE staining) and cell viability (PI staining). Groups were compared using an unpaired Student’s t test (p<0.05). Results: Groups did not differ regarding body weight, and sperm motility. Furthermore, air pollution did not alter sperm functional quality (Table 1). Conclusions: Exposure to high concentrations of PM2.5 does not affect sperm motility and functional parameters.
ISSN: 0015-0282
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