Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/59037
Title: Oxidative origin of sperm DNA fragmentation in the adult varicocele
Authors: Jeremias, Jessica Timóteo
Belardin, Larissa Berloffa
Okada, Fatima Kazue
Antoniassi, Mariana Pereira
Fraietta, Renato
Bertolla, Ricardo Pimenta
Intasqui, Paula
http://lattes.cnpq.br/5188240479960852
Keywords: DNA Fragmentation
Infertility, Male
Oxidative Stress
Varicocele
Issue Date: 10-Sep-2020
Publisher: Brazilian Society of Urology– SBU
Abstract: Purpose: Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. Materials and Methods: A cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student’s t test or a Mann-Whitney test. Results: The varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidativeinduced DNA fragmentation. Conclusions: Patients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage.
URI: https://repositorio.unifesp.br/handle/11600/59037
ISSN: 1677-6119
1677-5538
Other Identifiers: https://www.intbrazjurol.com.br/pdf/vol47n02/IBJU20190827.pdf
Appears in Collections:Artigo

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