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dc.contributor.authorMartins, Fernando H.
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.authorPiazza, Roxane M. F.
dc.contributor.authorElias, Waldir P.
dc.contributor.authorLeao, Sylvia Cardoso [UNIFESP]
dc.contributor.authorMarzoa, Juan
dc.contributor.authorDahbi, Ghizlane
dc.contributor.authorMora, Azucena
dc.contributor.authorBlanco, Miguel
dc.contributor.authorBlanco, Jorge
dc.contributor.authorPelayo, Jacinta S.
dc.identifier.citationVeterinary Microbiology. Amsterdam, v. 196, p. 72-77, 2016.
dc.description.abstractFood-producing animals can harbor Escherichia coli strains with potential to cause diseases in humans. In this study, the presence of enteropathogenic E. coli (EPEC) was investigated in fecal samples from 130 healthy sheep (92 lambs and 38 adults) raised for meat in southern Brazil. EPEC was detected in 19.2% of the sheep examined, but only lambs were found to be positive. A total of 25 isolates was characterized and designated atypical EPEC (aEPEC) as tested negative for bfpA gene and BFP production. The presence of virulence markers linked to human disease as ehxA, paa, and IpfAO(113) was observed in 60%, 24%, and 88% of the isolates, respectively. Of the 11 serotypes identified, eight were described among human pathogenic strains, while three (O1:H8, O11:H21 and O125:H19) were not previously detected in aEPEC. Associations between intimin subtypes and phylogroups were observed, including eae-theta 2/A, eae-beta 1/B1, eae-alpha 2/B2 and eae-gamma 1/D. Although PFGE typing of 16 aEPEC isolates resulted in 14 unique pulsetypes suggesting a genetic diversity, specific clones were found to be distributed in some flocks. In conclusion, potentially pathogenic aEPEC strains are present in sheep raised for meat, particularly in lambs, which can better contribute to dissemination of these bacteria than adult animals. (C) 2016 Published by Elsevier B.V.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipConselleria de Cultura, Education e Ordenacion Universitaria, Xunta de Galicia
dc.description.sponsorshipEuropean Regional Development Fund (ERDF)
dc.description.sponsorshipMinisterio de Economia y Competitividad, Gobierno de Espana
dc.publisherElsevier Science Bv
dc.relation.ispartofVeterinary Microbiology
dc.rightsAcesso restrito
dc.subjectFood productionen
dc.subjectVirulence factorsen
dc.titleLambs are an important source of atypical enteropathogenic Escherichia coli in southern Brazilen
dc.description.affiliationUniv Estadual Londrina, Dept Microbiol, Rodovia Celso Garcia Cid,PR-445 Km 380, Londrina, PR, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, SP, Brazil
dc.description.affiliationInst Butantan, Bacteriol Lab, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Santiago de Compostela, Fac Vet, Dept Microbiol & Parasitol, Lab Referencia E Coli, Lugo, Spain
dc.description.affiliationUnifespDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil
dc.description.sponsorshipIDERDF: CN2012/303
dc.description.sponsorshipIDMinisterio de Economia y Competitividad, Gobierno de Espana: AGL2013-47852-R
dc.description.sourceWeb of Science
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