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Title: Catalase expression impairs oxidative stress-mediated signalling in Trypanosoma cruzi
Authors: Guimaraes Freire, Anna Claudia
Alves, Ceres Luciana
Goes, Grazielle Ribeiro
Resende, Bruno Carvalho
Moretti, Nilmar Silvio [UNIFESP]
Nunes, Vinicius Santana [UNIFESP]
Nascimento Aguiar, Pedro Henrique
Tahara, Erich Birelli
Franco, Gloria Regina
Macedo, Andrea Mara
Junho Pena, Sergio Danilo
Gadelha, Fernanda Ramos
Guarneri, Alessandra Aparecida
Schenkman, Sergio [UNIFESP]
Vieira, Leda Quercia
Machado, Carlos Renato
Keywords: Trypanosoma cruzi
oxidative stress
oxidative signalling
Issue Date: 2017
Publisher: Cambridge Univ Press
Citation: Parasitology. New York, v. 144, n. 11, p. 1498-1510, 2017.
Abstract: Trypanosoma cruzi is exposed to oxidative stresses during its life cycle, and amongst the strategies employed by this parasite to deal with these situations sits a peculiar trypanothione-dependent antioxidant system. Remarkably, T. cruzi's antioxidant repertoire does not include catalase. In an attempt to shed light on what are the reasons by which this parasite lacks this enzyme, a T. cruzi cell line stably expressing catalase showed an increased resistance to hydrogen peroxide (H2O2) when compared with wild-type cells. Interestingly, preconditioning carried out with low concentrations of H2O2 led untransfected parasites to be as much resistant to this oxidant as cells expressing catalase, but did not induce the same level of increased resistance in the latter ones. Also, presence of catalase decreased trypanothione reductase and increased superoxide dismutase levels in T. cruzi, resulting in higher levels of residual H2O2 after challenge with this oxidant. Although expression of catalase contributed to elevated proliferation rates of T. cruzi in Rhodnius prolixus, it failed to induce a significant increase of parasite virulence in mice. Altogether, these results indicate that the absence of a gene encoding catalase in T. cruzi has played an important role in allowing this parasite to develop a shrill capacity to sense and overcome oxidative stress.
ISSN: 0031-1820
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