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dc.contributor.authorMartins, Fernando Henrique
dc.contributor.authorCabilio Guth, Beatriz Ernestina [UNIFESP]
dc.contributor.authorPiazza, Roxane Maria
dc.contributor.authorLeao, Sylvia Cardoso [UNIFESP]
dc.contributor.authorLudovico, Agostinho
dc.contributor.authorLudovico, Marilucia Santos
dc.contributor.authorDahbi, Ghizlane
dc.contributor.authorMarzoa, Juan
dc.contributor.authorMora, Azucena
dc.contributor.authorBlanco, Jorge
dc.contributor.authorPelayo, Jacinta Sanchez
dc.identifier.citationVeterinary Microbiology. Amsterdam: Elsevier B.V., v. 175, n. 1, p. 150-156, 2015.
dc.description.abstractSheep constitute an important source of zoonotic pathogens as Shiga toxin-producing Escherichia coli (STEC). in this study, the prevalence, serotypes and virulence profiles of STEC were investigated among 130 healthy sheep from small and medium farms in southern Brazil. STEC was isolated from 65 (50%) of the tested animals and detected in all flocks. A total of 70 STEC isolates were characterized, and belonged to 23 different O:H serotypes, many of which associated with human disease, including hemolytic-uremic syndrome (HUS). Among the serotypes identified, O76:H19 and O65:H- were the most common, and O75:H14 and O169:H7 have not been previously reported in STEC strains. Most of the STEC isolates harbored only stx1, whereas the Stx2b subtype was the most common among those carrying stx2. Enterohemolysin (ehxA) and intimin (eae) genes were detected in 61 (87.1%) and four (5.7%) isolates, respectively. Genes encoding putative adhesins (saa, iha, lpf(O113)) and toxins (subAB and cdtV) were also observed. the majority of the isolates displayed virulence features related to pathogenesis of STEC, such as adherence to epithelial cells, high cytotoxicity and enterohemolytic activity. Ovine STEC isolates belonged mostly to phylogenetic group B1. PFGE revealed particular clones distributed in some farms, as well as variations in the degree of genetic similarity within serotypes examined. in conclusion, STEC are widely distributed in southern Brazilian sheep, and belonged mainly to serotypes that are not commonly reported in other regions, such as 076:H19 and 065:H-. A geographical variation in the distribution of STEC serotypes seems to occur in sheep. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipConselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia
dc.description.sponsorshipEuropean Regional Development Fund (ERDF)
dc.description.sponsorshipMinisterio de Economia y Competitividad, Gobierno de Espana
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Microbiology
dc.rightsAcesso restrito
dc.subjectShiga toxin-producing E. colien
dc.subjectVirulence factorsen
dc.subjectGenetic diversityen
dc.titleDiversity of Shiga toxin-producing Escherichia coli in sheep flocks of Parana State, southern Brazilen
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniv Norte Parana
dc.description.affiliationUniv Estadual Londrina, Dept Microbiol, Londrina, PR, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Bacteriol, São Paulo, Brazil
dc.description.affiliationUniv Norte Parana, Fac Med Vet, Arapongas, PR, Brazil
dc.description.affiliationUSC, LREC, Dept Microbiol & Parasitol, Fac Vet, Lugo, Spain
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sponsorshipIDEuropean Regional Development Fund (ERDF): CN2012/303
dc.description.sponsorshipIDMinisterio de Economia y Competitividad, Gobierno de Espana: AGL2013-47852-R
dc.description.sourceWeb of Science
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