Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/36167
Full metadata record
DC FieldValueLanguage
dc.contributor.authorVaz, Antonio F. M.
dc.contributor.authorSouza, Marthyna P.
dc.contributor.authorVieira, Leucio D.
dc.contributor.authorAguiar, Jaciana S.
dc.contributor.authorSilva, Teresinha G.
dc.contributor.authorMedeiros, Paloma L.
dc.contributor.authorMelo, Ana M. M. A.
dc.contributor.authorSilva-Lucca, Rosemeire A. [UNIFESP]
dc.contributor.authorSantana, Lucimeire A. [UNIFESP]
dc.contributor.authorOliva, Maria L. V. [UNIFESP]
dc.contributor.authorPerez, Katia R. [UNIFESP]
dc.contributor.authorCuccovia, Iolanda M.
dc.contributor.authorCoelho, Luana C. B. B.
dc.contributor.authorCorreia, Maria T. S.
dc.date.accessioned2016-01-24T14:31:31Z-
dc.date.available2016-01-24T14:31:31Z-
dc.date.issued2013-04-01
dc.identifierhttp://dx.doi.org/10.1016/j.radphyschem.2012.12.015
dc.identifier.citationRadiation Physics and Chemistry. Oxford: Pergamon-Elsevier B.V., v. 85, p. 218-226, 2013.
dc.identifier.issn0969-806X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36167-
dc.description.abstractOne of the most promising areas for the development of functional foods lies in the development of effective methods to reduce or eliminate food allergenicity, but few reports have summarized information concerning the progress made with food irradiation. in this study, we investigated the relationship between allergenicity and molecular structure of a food allergen after gamma irradiation and evaluate the profile of the allergic response to irradiated allergens. Cramoll, a lectin isolated from a bean and used as a food allergen, was irradiated and the possible structural changes were accompanied by spectrofluorimetry, circular dichroism and microcalorimetry. Subsequently, sensitized animals subjected to intragastric administration of non-irradiated and irradiated Cramoll were treated for 7 days. Then, body weight, leukocytes, cytokine profiles and histological parameters were also determined. Cramoll showed complete inhibition of intrinsic activity after high radiation doses. Changes in fluorescence and CD spectra with a simultaneous collapse of the tertiary structure followed by a pronounced decrease of native secondary structure were observed after irradiation. After oral challenge, sensitized mice demonstrate an association between Cramoll intake, body weight loss, eosinophilia, lymphocytic infiltrate in the gut and Eotaxin secretion. Irradiation significantly reduces, according to the dose, the effects observed by non-irradiated food allergens. We confirm that high-dose radiation may render protein food allergens innocuous by irreversibly compromising their molecular structure. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipMinisterio da Ciencia e Tecnologia (Brazilian)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundacao de Amparo a Ciencia e Tecnologia do estado de Pernambuco (FACEPE)
dc.format.extent218-226
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRadiation Physics and Chemistry
dc.rightsAcesso restrito
dc.subjectFood allergyen
dc.subjectFood allergenen
dc.subjectFood irradiationen
dc.subjectLectinen
dc.titleHigh doses of gamma radiation suppress allergic effect induced by food lectinen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniv Fed Campina Grande
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniv Estadual Oeste Parana
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUniv Fed Campina Grande, Ctr Saude & Tecnol Rural, BR-58700970 Patos de Minas, Paraiba, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Bioquim, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Fisiol & Farmacol, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Antibiot, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Histol & Embriol, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Biofis & Radiobiol, Recife, PE, Brazil
dc.description.affiliationUniv Estadual Oeste Parana, Ctr Engn & Ciencias Exatas, Toledo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim, Inst Quim, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.identifier.doi10.1016/j.radphyschem.2012.12.015
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000317030000034
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.