Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/32433
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCosta, Romero M. P. B.
dc.contributor.authorVaz, Antonio F. M.
dc.contributor.authorOliva, Maria L. V. [UNIFESP]
dc.contributor.authorCoelho, Luana C. B. B.
dc.contributor.authorCorreia, Maria T. S.
dc.contributor.authorCarneiro-da-Cunha, Maria G.
dc.date.accessioned2016-01-24T13:59:32Z-
dc.date.available2016-01-24T13:59:32Z-
dc.date.issued2010-04-01
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2009.11.013
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 45, n. 4, p. 526-533, 2010.
dc.identifier.issn1359-5113
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32433-
dc.description.abstractPhthirusa pyrifolia leaf lectin (PpyLL) was obtained from the hemi-parasitic medicinal plant mistletoe through saline saturation and two consecutive chromatographic steps on Sephadex G-100 and ion exchange on CM-cellulose. SDS-PAGE of the protein under non-reducing conditions revealed a monomeric protein with a molecular weight of 15.6 kDa. Under reducing conditions in the presence of 2-mercaptoethanol, the protein showed two bands with molecular weights of 15 and 7 kDa. PpyLL, an acidic glycoprotein with 19% sugar content, was not dependent on divalent cations. It was stable up to 70 degrees C and exhibited maximum hemagglutination at pH 7.5. Lectin fluorescence emission spectra at different temperatures showed that the lectin fluorescence increased when the temperature increased. PpyLL showed a unique affinity for the phosphate derivative of fructose, fructose-1-6-biphosphate. PpyLL showed effective antimicrobial activities against bacteria (Gram-positive: Staphylococcus epidermidis. Streptococcus faecalis and Bacillus subtilis; Gram-negative: Klebsiella pneumoniae) and fungi (Fusarium lateritium and Rhizoctonia solani). Therefore, PpyLL specificity, as determined by a new sugar affinity, may be significant to determine its biological potential. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent526-533
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProcess Biochemistry
dc.rightsAcesso restrito
dc.subjectLectinen
dc.subjectPhthirusa pyrifoliaen
dc.subjectMistletoeen
dc.subjectChromatographyen
dc.subjectFluorescenceen
dc.subjectAntimicrobial activityen
dc.titleA new mistletoe Phthirusa pyrifolia leaf lectin with antimicrobial propertiesen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniv Fed Pernambuco, Ctr Ciencias Biol, Dept Bioquim, BR-50670420 Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, LIKA, BR-50670901 Recife, PE, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipIDCAPES: 1415/2007
dc.identifier.doi10.1016/j.procbio.2009.11.013
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000276376900015
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.