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dc.contributor.authorCosta, Romero M. P. B.
dc.contributor.authorVaz, Antonio F. M.
dc.contributor.authorOliva, Maria L. V. [UNIFESP]
dc.contributor.authorCoelho, Luana C. B. B.
dc.contributor.authorCorreia, Maria T. S.
dc.contributor.authorCarneiro-da-Cunha, Maria G.
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 45, n. 4, p. 526-533, 2010.
dc.description.abstractPhthirusa pyrifolia leaf lectin (PpyLL) was obtained from the hemi-parasitic medicinal plant mistletoe through saline saturation and two consecutive chromatographic steps on Sephadex G-100 and ion exchange on CM-cellulose. SDS-PAGE of the protein under non-reducing conditions revealed a monomeric protein with a molecular weight of 15.6 kDa. Under reducing conditions in the presence of 2-mercaptoethanol, the protein showed two bands with molecular weights of 15 and 7 kDa. PpyLL, an acidic glycoprotein with 19% sugar content, was not dependent on divalent cations. It was stable up to 70 degrees C and exhibited maximum hemagglutination at pH 7.5. Lectin fluorescence emission spectra at different temperatures showed that the lectin fluorescence increased when the temperature increased. PpyLL showed a unique affinity for the phosphate derivative of fructose, fructose-1-6-biphosphate. PpyLL showed effective antimicrobial activities against bacteria (Gram-positive: Staphylococcus epidermidis. Streptococcus faecalis and Bacillus subtilis; Gram-negative: Klebsiella pneumoniae) and fungi (Fusarium lateritium and Rhizoctonia solani). Therefore, PpyLL specificity, as determined by a new sugar affinity, may be significant to determine its biological potential. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.publisherElsevier B.V.
dc.relation.ispartofProcess Biochemistry
dc.rightsAcesso restrito
dc.subjectPhthirusa pyrifoliaen
dc.subjectAntimicrobial activityen
dc.titleA new mistletoe Phthirusa pyrifolia leaf lectin with antimicrobial propertiesen
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniv Fed Pernambuco, Ctr Ciencias Biol, Dept Bioquim, BR-50670420 Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, LIKA, BR-50670901 Recife, PE, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipIDCAPES: 1415/2007
dc.description.sourceWeb of Science
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