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Title: A new mistletoe Phthirusa pyrifolia leaf lectin with antimicrobial properties
Authors: Costa, Romero M. P. B.
Vaz, Antonio F. M.
Oliva, Maria L. V. [UNIFESP]
Coelho, Luana C. B. B.
Correia, Maria T. S.
Carneiro-da-Cunha, Maria G.
Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Lectin
Phthirusa pyrifolia
Antimicrobial activity
Issue Date: 1-Apr-2010
Publisher: Elsevier B.V.
Citation: Process Biochemistry. Oxford: Elsevier B.V., v. 45, n. 4, p. 526-533, 2010.
Abstract: Phthirusa pyrifolia leaf lectin (PpyLL) was obtained from the hemi-parasitic medicinal plant mistletoe through saline saturation and two consecutive chromatographic steps on Sephadex G-100 and ion exchange on CM-cellulose. SDS-PAGE of the protein under non-reducing conditions revealed a monomeric protein with a molecular weight of 15.6 kDa. Under reducing conditions in the presence of 2-mercaptoethanol, the protein showed two bands with molecular weights of 15 and 7 kDa. PpyLL, an acidic glycoprotein with 19% sugar content, was not dependent on divalent cations. It was stable up to 70 degrees C and exhibited maximum hemagglutination at pH 7.5. Lectin fluorescence emission spectra at different temperatures showed that the lectin fluorescence increased when the temperature increased. PpyLL showed a unique affinity for the phosphate derivative of fructose, fructose-1-6-biphosphate. PpyLL showed effective antimicrobial activities against bacteria (Gram-positive: Staphylococcus epidermidis. Streptococcus faecalis and Bacillus subtilis; Gram-negative: Klebsiella pneumoniae) and fungi (Fusarium lateritium and Rhizoctonia solani). Therefore, PpyLL specificity, as determined by a new sugar affinity, may be significant to determine its biological potential. (C) 2009 Elsevier B.V. All rights reserved.
ISSN: 1359-5113
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