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|Title:||Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells|
|Authors:||Vieira, Monica A. M. [UNIFESP]|
Gomes, Tania A. T. [UNIFESP]
Ferreira, Antonio J. P.
Servin, Alain L.
Lievin-Le Moal, Vanessa
Univ Paris 11
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
|Publisher:||Amer Soc Microbiology|
|Citation:||Infection and Immunity. Washington: Amer Soc Microbiology, v. 78, n. 3, p. 927-938, 2010.|
|Abstract:||In rabbit ligated ileal loops, two atypical enteropathogenic Escherichia coli (aEPEC) strains, 3991-1 and 0421-1, intimately associated with the cell membrane, forming the characteristic EPEC attachment and effacement lesion of the brush border, induced a mucous hypersecretion, whereas typical EPEC (tEPEC) strain E2348/69 did not. Using cultured human mucin-secreting intestinal HT29-MTX cells, we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. the transcription of the MUC5AC and MUC4 genes was transiently upregulated after aEPEC infection. We provide evidence that the apically adhering aEPEC cells exploit the mucins' increased production since they grew in the presence of membrane-bound mucins, whereas tEPEC did not. the data described herein report a putative new virulence phenomenon in aEPEC.|
|Appears in Collections:||Em verificação - Geral|
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