Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/31413
Title: Characterization of the Trypanosoma cruzi ortholog of the SBDS protein reveals an intrinsically disordered extended C-terminal region showing RNA-interacting activity
Authors: Oliveir, Juliana Ferreira de
Castilho, Beatriz A. [UNIFESP]
Sforca, Mauricio L.
Krieger, Marco Aurelio
Zeri, Ana Carolina
Guimaraes, Beatriz G.
Zanchin, Nilson I. T.
LNLS
Universidade Federal de São Paulo (UNIFESP)
IBMP
Keywords: SBDS ortholog
Trypanosoma cruzi
RNA-protein interaction
Protein structure
Issue Date: 1-Apr-2009
Publisher: Elsevier B.V.
Citation: Biochimie. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 91, n. 4, p. 475-483, 2009.
Abstract: The human SBDS gene and its yeast ortholog SDOI encode essential proteins that are involved in ribosome biosynthesis. SDOI has been implicated in recycling of the ribosomal biogenesis factor Tif6p from pre-66S particles as well as in translation activation of 60S ribosomes. the SBDS protein is highly conserved, containing approximately 250 amino acid residues in animals, fungi and Archaea, while SBDS orthologs of plants and a group of protists contain an extended C-terminal region. in this work, we describe the characterization of the Trypanosoma cruzi SBDS ortholog (TcSBDS). TcSBDS co-fractionates with polysomes in sucrose density gradients, which is consistent with a role in ribosome biosynthesis. We show that TcSBDS contains a C-terminal extension of 200 amino acids that displays the features of intrinsically disordered proteins as determined by proteolytic, circular dichroism and NMR analyses. Interestingly, the C-terminal extension is responsible for TcSBDS-RNA interaction activity in electrophoretic mobility shift assays. This finding suggests that Trypanosomatidae and possibly also other organisms containing SBDS with extended C-terminal regions have evolved an additional function for SBDS in ribosome biogenesis. (C) 2008 Elsevier Masson SAS. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/31413
ISSN: 0300-9084
Other Identifiers: http://dx.doi.org/10.1016/j.biochi.2008.12.001
Appears in Collections:Em verificação - Geral

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