Toll like receptors gene expression of human keratinocytes cultured of severe burn injury

Toll like receptors gene expression of human keratinocytes cultured of severe burn injury

Author Cornick, Sarita Mac Google Scholar
Corrêa, Silvana Aparecida Alves Autor UNIFESP Google Scholar
Noronha, Samuel Marcos Ribeiro de Google Scholar
Cezillo, Marcus V. B. Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Gragnani, Alfredo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To evaluate the expression profile of genes related to Toll Like Receptors (TLR) pathways of human Primary Epidermal keratinocytes of patients with severe burns.METHODS: After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol(r) (Life Technologies) for extraction of total RNA. This was quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using specific TLR pathways PCR Arrays plates (SA Biosciences).RESULTS: After the analysis of gene expression we found that 21% of these genes were differentially expressed, of which 100% were repressed or hyporegulated. Among these, the following genes (fold decrease): HSPA1A (-58), HRAS (-36), MAP2K3 (-23), TOLLIP (-23), RELA (-18), FOS (-16), and TLR1 (-6.0).CONCLUSIONS: This study contributes to the understanding of the molecular mechanisms related to TLR pathways and underlying wound infection caused by the burn. Furthermore, it may provide new strategies to restore normal expression of these genes and thereby change the healing process and improve clinical outcome.
Keywords Gene Expression
Burn
Toll Like Receptors
Human Keratinocytes
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2011/12945-4
FAPESP: 2013/10.905-0
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 33-38, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 33-38
Origin http://dx.doi.org/10.1590/S0102-86502014001700007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344466300008
SciELO ID S0102-86502014001500033 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8135

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