Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Author Watanabe, Aripuanã Sakurada Aranha Autor UNIFESP Google Scholar
Carraro, Emerson Autor UNIFESP Google Scholar
Camargo, Clarice Neves Autor UNIFESP Google Scholar
Puerari, Diane Google Scholar
Guatura, Sandra Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Bellei, Nancy Cristina Junqueira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade do Centro-Oeste Departamento de farmacologia Clínica
Abstract INTRODUCTION: Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different methods: direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset. METHODS: Samples (n=643) were collected from different risk groups during from 2001 to 2010: 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program. RESULTS: Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR: 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection. CONCLUSIONS: Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service.
Keywords HAdV
Respiratory infection
DFA
Nested PCR
Immunocompromised patients and immunocompetent patients
Language English
Date 2013-04-09
Published in Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 161-165, 2013.
ISSN 0037-8682 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Medicina Tropical - SBMT
Extent 161-165
Origin http://dx.doi.org/10.1590/0037-8682-1699-2013
Access rights Open access Open Access
Type Article
SciELO ID S0037-86822013000200161 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7732

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