Human TNF-alpha induces differential protein phosphorylation in Schistosoma mansoni adult male worms

Human TNF-alpha induces differential protein phosphorylation in Schistosoma mansoni adult male worms

Author Oliveira, Katia C. Autor UNIFESP Google Scholar
Carvalho, Mariana L. P. Google Scholar
Bonatto, Jose Matheus C. Google Scholar
Schechtman, Debora Google Scholar
Verjovski-Almeida, Sergio Google Scholar
Abstract Schistosoma mansoni and its vertebrate host have a complex and intimate connection in which several molecular stimuli are exchanged and affect both organisms. Human tumor necrosis factor alpha (hTNF-alpha), a pro-inflammatory cytokine, is known to induce large-scale gene expression changes in the parasite and to affect several parasite biological processes such as metabolism, egg laying, and worm development. Until now, the molecular mechanisms for TNF-alpha activity in worms are not completely understood. Here, we aimed at exploring the effect of hTNF-alpha on S. mansoni protein phosphorylation by 2D gel electrophoresis followed by a quantitative analysis of phosphoprotein staining and protein identification by mass spectrometry. We analyzed three biological replicates of adult male worms exposed to hTNF-alpha and successfully identified 32 protein spots with a statistically significant increase in phosphorylation upon in vitro exposure to hTNF-alpha. Among the differentially phosphorylated proteins, we found proteins involved in metabolism, such as glycolysis, galactose metabolism, urea cycle, and aldehyde metabolism, as well as proteins related to muscle contraction and to cytoskeleton remodeling. The most differentially phosphorylated protein (30-fold increase in phosphorylation) was 14-3-3, whose function is known to be modulated by phosphorylation, belonging to a signal transduction protein family that regulates a variety of processes in all eukaryotic cells. Further, 75 % of the identified proteins are known in mammals to be related to TNF-alpha signaling, thus suggesting that TNF-alpha response may be conserved in the parasite. We propose that this work opens new perspectives to be explored in the study of the molecular crosstalk between host and pathogen.
Keywords Schistosoma mansoni
Human TNF-alpha
Phosphoproteomic analysis
Mass spectrometry
Host-parasite interaction
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Date 2016
Published in Parasitology Research. New York, v. 115, n. 2, p. 817-828, 2016.
ISSN 0932-0113 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 817-828
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000370868800041

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