Subtractive phage display selection for screening and identification of peptide sequences with potential use in serodiagnosis of paracoccidioidomycosis caused by Paracoccidioides brasiliensis

Subtractive phage display selection for screening and identification of peptide sequences with potential use in serodiagnosis of paracoccidioidomycosis caused by Paracoccidioides brasiliensis

Author da Silva Portes, L. Autor UNIFESP Google Scholar
Kioshima, E. S. Google Scholar
de Camargo, Z. P. Autor UNIFESP Google Scholar
Batista, W. L. Autor UNIFESP Google Scholar
Xander, P. Autor UNIFESP Google Scholar
Abstract Paracoccidioidomycosis (PCM) is a systemic granulomatous disease endemic in Latin America whose aetiologic agents are the thermodimorphic fungi Paracoccidioides brasiliensis and Paracoccidioides lutzii. Despite technological advances, some problems have been reported for the fungal antigens used for serological diagnosis, and inconsistencies among laboratories have been reported. The use of synthetic peptides in the serological diagnosis of infectious diseases has proved to be a valuable strategy because in some cases, the reactions are more specific and sensitive. In this study, we used a subtractive selection with a phage display library against purified polyclonal antibodies for negative and positive PCM sera caused by P. brasiliensis. The binding phages were sequenced and tested in a binding assay to evaluate its interaction with sera from normal individuals and PCM patients. Synthetic peptides derived from these phage clones were tested in a serological assay, and we observed a significant recognition of LP15 by sera from PCM patients infected with P. brasiliensis. Our results demonstrated that subtractive phage display selection may be useful for identifying new epitopes that can be applied to the serodiagnosis of PCM caused by P. brasiliensis.
Keywords ELISA
paracoccidioidomycosis
phage display
serodiagnosis
synthetic peptides
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Date 2017
Published in Letters In Applied Microbiology. Hoboken, v. 65, n. 5, p. 346-353, 2017.
ISSN 0266-8254 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 346-353
Origin http://dx.doi.org/10.1111/lam.12788
Access rights Closed access
Type Article
Web of Science ID WOS:000417427700001
URI https://repositorio.unifesp.br/handle/11600/58232

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