EVALUATION OF EXPERIMENTAL INFECTION WITH L. (L.) AMAZONENSIS IN X-LINKED IMMUNODEFICIENT MICE

EVALUATION OF EXPERIMENTAL INFECTION WITH L. (L.) AMAZONENSIS IN X-LINKED IMMUNODEFICIENT MICE

Author Gonzaga, Wagner Francisco Kennerly Marcondes Autor UNIFESP Google Scholar
Geraldo, Mariana Marques Autor UNIFESP Google Scholar
Vivanco, Bruno Camolese Autor UNIFESP Google Scholar
Popi, Ana Flavia Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Batista, Wagner Luiz Autor UNIFESP Google Scholar
Xander, Patricia Autor UNIFESP Google Scholar
Abstract B-1 cells are a subtype of B cells with peculiar characteristics. These cells are distinct from B-2 lymphocytes in their morphology, ontogeny, tissue distribution, and phenotypic functional features. B-1 cells can participate in the immune response in several ways, for example, by being recruited to inflammatory foci, producing large amounts of IL-10 cytokine, and differentiating into IgM-secreting cells or phagocytes. Nevertheless, the role of B-1 cells in the pathogenesis of experimental leishmaniasis has not been fully elucidated. Here we evaluated the role of B-1 cells in Leishmania (L.) amazonensis infection using X-linked immunodeficient (XID) mice that possess a mutation in Bruton's tyrosine kinase (Btk) that leads to a reduced number of B-1 cells. The course of infection and the corresponding immune response were analyzed in infected mice. XID mice showed an increase in parasite number in paws, lymph nodes, and spleen compared to BALB/c infected controls. Infected XID mice had higher IL-10 levels and lower anti-Leishmania IgM. The adoptive transfer of peritoneal B-1 cells into XID mice restored peritoneal B-1 cells and parasite burden in the footpad in a pattern similar to that observed in the BALB/c controls at 10 wk. Our results demonstrate the higher susceptibility of XID mice to infection with L. (L.) amazonensis compared to controls. In addition, we show that the presence of B-1 cells contributes to improved animal resistance to parasites, suggesting that these cells are involved in the control of cutaneous infection caused by L. (L.) amazonensis.
xmlui.dri2xhtml.METS-1.0.item-coverage Lawrence
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Coordenacao de Aperfeicoamento Pessoal de Ensino Superior (CAPES)
Grant number FAPESP: 2012/11837-6
Date 2017
Published in Journal Of Parasitology. Lawrence, v. 103, n. 6, p. 708-717, 2017.
ISSN 0022-3395 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Parasitologists
Extent 708-717
Origin http://dx.doi.org/10.1645/16-145
Access rights Closed access
Type Article
Web of Science ID WOS:000419232400016
URI https://repositorio.unifesp.br/handle/11600/58057

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