Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization

Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization

Author Okada, Sabrina Sayori Google Scholar
de Oliveira, Edson Mendes Google Scholar
de Araujo, Tomaz Henrique Google Scholar
Rodrigues, Maria Rita Google Scholar
Albuquerque, Renata Chaves Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Taniwaki, Noemi Nosomi Google Scholar
Nakaya, Helder Imoto Google Scholar
Campa, Ana Google Scholar
Ramos Moreno, Ana Carolina Google Scholar
Abstract Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms. In peripheral blood, it is accepted that MPO is only produced by myeloid-lineage cells. Thus, MPO presence is unexpected in lymphocytes. We showed recently that BI-lymphocytes from mice have MPO. Here, we showed that subsets of human peripheral B, CD4(+) and CD8(+) T lymphocytes express MPO. The content of MPO in lymphocytes was very low compared to neutrophils/monocytes with a preferential distribution in the nucleus and perinuclear region. Also, we performed a MPO mRNA expression analysis from human blood cells derived from microarray raw data publicly available, showing that MPO is modulated in infectious disease. MPO was increased in CD4(+) T lymphocytes from HIV chronic infection and in CD8(+) T lymphocytes from HCV-positive patients. Our study points out MPO as a multifunctional protein due to its subcellular localization and expression modulation in lymphocytes indicating alternative unknown functions for MPO in lymphocytes. (C) 2015 Elsevier Inc. All rights reserved.
Keywords Electron microscopy
FACS
Fluorescence microscopy
Moonlighting protein
Subcellular localization
xmlui.dri2xhtml.METS-1.0.item-coverage San Diego
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq
Grant number FAPESP: 2009/14632-3
FAPESP: 2008/54474-5
FAPESP: 06/50641-9
FAPESP: 2010/18498-7
CNPq: 47151012010-6
Date 2016
Published in Cellular Immunology. San Diego, v. 300, p. 18-25, 2016.
ISSN 0008-8749 (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Elsevier Science
Extent 18-25
Origin http://dx.doi.org/10.1016/j.cellimm.2015.11.003
Access rights Closed access
Type Article
Web of Science ID WOS:000371191600003
URI https://repositorio.unifesp.br/handle/11600/58008

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