Antibodies Against Glycolipids Enhance Antifungal Activity of Macrophages and Reduce Fungal Burden After Infection with Paracoccidioides brasiliensis

Antibodies Against Glycolipids Enhance Antifungal Activity of Macrophages and Reduce Fungal Burden After Infection with Paracoccidioides brasiliensis

Author Bueno, Renata A. Google Scholar
Thomaz, Luciana Google Scholar
Munoz, Julian E. Google Scholar
da Silva, Cassia J. Google Scholar
Nosanchuk, Joshua D. Google Scholar
Pinto, Marcia R. Google Scholar
Travassos, Luiz R. Autor UNIFESP Google Scholar
Taborda, Carlos P. Google Scholar
Abstract Paracoccidioidomycosis is a fungal disease endemic in Latin America. Polyclonal antibodies to acidic glycosphingolipids (GSLs) from Paracoccidioides brasiliensis opsonized yeast forms in vitro increasing phagocytosis and reduced the fungal burden of infected animals. Antibodies to GSL were active in both prophylactic and therapeutic protocols using a murine intratracheal infection model. Pathological examination of the lungs of animals treated with antibodies to GSL showed well-organized granulomas and minimally damaged parenchyma compared to the untreated control. Murine peritoneal macrophages activated by IFN-gamma and incubated with antibodies against acidic GSLs more effectively phagocytosed and killed P brasiliensis yeast cells as well as produced more nitric oxide compared to controls. The present work discloses a novel target of protective antibodies against P brasiliensis adding to other well-studied mediators of the immune response to this fungus.
Keywords Paracoccidioides brasiliensis
paracoccidioidomycosis
glycosphingolipids
polyclonal antibodies
nitric oxide
xmlui.dri2xhtml.METS-1.0.item-coverage Lausanne
Language English
Sponsor Capes
FAPESP
Grant number FAPESP: 2011/17267-4
FAPESP: 2013/18655-3
Date 2016
Published in Frontiers In Microbiology. Lausanne, v. 7, p. -, 2016.
ISSN 1664-302X (Sherpa/Romeo, impact factor)
Publisher Frontiers Media Sa
Extent -
Origin http://dx.doi.org/10.3389/fmicb.2016.00074
Access rights Closed access
Type Article
Web of Science ID WOS:000369134400001
URI https://repositorio.unifesp.br/handle/11600/57985

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