Protective effects of mito-TEMPO against doxorubicin cardiotoxicity in mice

Protective effects of mito-TEMPO against doxorubicin cardiotoxicity in mice

Author Junqueira Rocha, Viviane Costa Google Scholar
de Aragao Franca, Luciana Souza Google Scholar
de Araujo, Cintia Figueiredo Google Scholar
Ng, Ayling Martins Google Scholar
de Andrade, Candace Machado Google Scholar
Andrade, Andre Cronemberger Autor UNIFESP Google Scholar
Santos, Emanuelle de Souza Google Scholar
Borges-Silva, Mariana da Cruz Google Scholar
Macambira, Simone Garcia Google Scholar
Noronha-Dutra, Alberto Augusto Google Scholar
Pontes-de-Carvalho, Lain Carlos Google Scholar
Abstract Doxorubicin (DOX) is a chemotherapeutic that is widely used for the treatment of many human tumors. However, the development of cardiotoxicity has limited its use. The aim of the present study was to evaluate the possible efficacy of mito-TEMPO (mito-T) as a protective agent against DOX-induced cardiotoxicity in mice. C57BL/6 mice were treated twice with mito-T at low (5 mg/kg body weight) or high (20 mg/kg body weight) dose and once with DOX (24 mg/kg body weight) or saline (0.1 mL/20 g body weight) by means of intraperitoneal injections. The levels of malondialdehyde (MLDA), a marker of lipid peroxidation, and serum levels of creatine kinase were evaluated 48 h after the injection of DOX. DOX induced lipid peroxidation in heart mitochondria (p < 0.001), and DOX-treated mice receiving mito-T at low dose had levels of MLDA significantly lower than the mice that received only DOX (p < 0.01). Furthermore, administration of mito-T alone did not cause any significant changes from control values. Additionally, DOX-treated mice treated with mito-T at high dose showed decrease in serum levels of total CK compared to mice treated with DOX alone (p < 0.05). Our results indicate that mito-T protects mice against DOX-induced cardiotoxicity.
Keywords Doxorubicin
Cardiotoxicity
Mitochondria
Mito-TEMPO
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado da Bahia-FAPESB, State Government of Bahia, Brazil
Fundacao Oswaldo Cruz, Brazil
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq, Ministry of Science and Technology, Brazil
Date 2016
Published in Cancer Chemotherapy And Pharmacology. New York, v. 77, n. 3, p. 659-662, 2016.
ISSN 0344-5704 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 659-662
Origin http://dx.doi.org/10.1007/s00280-015-2949-7
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000371246100023
URI https://repositorio.unifesp.br/handle/11600/57908

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