Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

Author Souza, Janaina Sena de Autor UNIFESP Google Scholar
Brunetto, Erika L. Google Scholar
Nunes, Maria Tereza Google Scholar
Abstract Iron is an important trace element for proper cell functioning. It is present in cytochromes, hemoglobin and myoglobin (Mb), where it binds to oxygen. It is also an electron acceptor in the respiratory chain. Mb is an 18 kDa heme-protein, highly expressed in skeletal muscle and heart. The expression of several genes involved in the metabolism of iron is post-transcriptionally regulated by this element. Iron was shown to interfere with the polyadenylation step, modifying their poly (A) tail length and, as a consequence, their stability and translation rate. The aim of this study was to investigate whether iron supplementation or long and short-term restriction affects Mb gene and protein expression, as well as Mb mRNA poly(A) tail length, in cardiac and skeletal muscles of rats. Long-term iron restriction caused an increase in Mb gene and protein expression in Soleus muscle. No changes were observed in extensor digitorum longus muscle and heart. Short-term iron supplementation after iron deprivation did not alter Mb gene expression and mRNA poly(A) tail length in all tissues studied. These results indicate that Mb gene and protein expression is upregulated in response to iron deprivation, an effect that is tissue-specific and seems to occur at transcriptional level.
Keywords cardiac muscle
iron
Myoglobin
skeletal muscle
xmlui.dri2xhtml.METS-1.0.item-coverage Rio Janeiro
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 07/6096-5
CNPq: 305659/2009-0
Date 2016
Published in Anais Da Academia Brasileira De Ciencias. Rio Janeiro, v. 88, n. 4, p. 2277-2290, 2016.
ISSN 0001-3765 (Sherpa/Romeo, impact factor)
Publisher Acad Brasileira De Ciencias
Extent 2277-2290
Origin http://dx.doi.org/10.1590/0001-3765201620160173
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000391581800020
SciELO ID S0001-37652016000602277 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/56945

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