CD163 and CD206 expression does not correlate with tolerance and cytokine production in LPS-tolerant human monocytes

CD163 and CD206 expression does not correlate with tolerance and cytokine production in LPS-tolerant human monocytes

Author Alves-Januzzi, Amanda Barba Autor UNIFESP Google Scholar
Colo Brunialti, Milena Karina Autor UNIFESP Google Scholar
Salomao, Reinaldo Autor UNIFESP Google Scholar
Abstract BackgroundLipopolysaccharide (LPS)-tolerant monocytes produce small amounts of inflammatory cytokines, which is one of the characteristics of the alternative activated macrophages (AAM). These cells exhibited an increased expression of CD206 and CD163. Given the functional similarities of AAMs with the modulation of monocytes' functions observed during sepsis and LPS-tolerance, we evaluated whether the inhibition of inflammatory cytokine production by LPS-tolerant monocytes is associated with the phenotype of cells expressing CD206 and CD163. MethodsWe investigated whether tolerant human monocytes would modulate their expression of CD206 and CD163, markers of alternative activation, and whether the level of their expression would be related to cytokines detection. Tolerance to LPS was induced in peripheral blood mononuclear cell by pre-incubating the cells with increasing concentrations of LPS. The expression of CD206 and CD163 and intracellular TNF- and IL-6 was determined 24 h after LPS challenge by flow cytometry. ResultsNo differences in CD163 expression were observed between tolerant and non-tolerant cells, while the expression of CD206, which was decreased following LPS stimulation in non-tolerized cells, was further reduced in tolerant cells. Decreased production of inflammatory cytokines was observed in the tolerized cells, regardless of the expression of CD163 and CD206, with the exception of IL-6 in CD206+ monocytes, which was similarly expressed in both tolerized and non-tolerized cells. ConclusionsThe effect of LPS in the expression of CD163 and CD206 on monocytes is not reverted in LPS tolerant cells, and the inhibition of inflammatory cytokines in tolerant cells is not related with modulation of these receptors. (c) 2016 International Clinical Cytometry Society
Keywords LPS tolerance
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Grant number FAPESP: 2011/20401-4
Date 2017
Published in Cytometry Part B-Clinical Cytometry. Hoboken, v. 92, n. 3, p. 192-199, 2017.
ISSN 1552-4949 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 192-199
Access rights Closed access
Type Article
Web of Science ID WOS:000401342000004

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