Characterization of Enterococcus species isolated from marine recreational waters by MALDI-TOF MS and Rapid ID API (R) 20 Strep system

Characterization of Enterococcus species isolated from marine recreational waters by MALDI-TOF MS and Rapid ID API (R) 20 Strep system

Author Guarnieri Christ, Ana Paula Google Scholar
Ramos, Solange Rodrigues Google Scholar
Cayo, Rodrigo Autor UNIFESP Google Scholar
Gales, Ana Cristina Autor UNIFESP Google Scholar
Hachich, Elayse Maria Google Scholar
Zanoli Sato, Maria Ines Google Scholar
Abstract MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMerieux's API (R) 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods. The 165 rRNA gene sequencing of isolates with discrepant results showed that MALDI-TOF and API (R) correctly identified 74% and 11% of these isolates, respectively. This discrepancy probably relies on the bias of the API (R) has to identify clinical isolates. MALDI-TOF proved to be a feasible approach for identifying Enterococcus from environmental matrices increasing the rapidness and accuracy of results. (C) 2017 Elsevier Ltd. All rights reserved.
Keywords Enterococci
Species identification
Gram-positive
Beaches
16S rRNA gene sequencing
MALDI-TOF MS
xmlui.dri2xhtml.METS-1.0.item-coverage Oxford
Language English
Sponsor CETESB - Environmental Company of sao Paulo State
CNPq - National Council for Science and Technological Development
Grant number CNPq: 305535/2014-5
Date 2017
Published in Marine Pollution Bulletin. Oxford, v. 118, n. 43497, p. 376-381, 2017.
ISSN 0025-326X (Sherpa/Romeo, impact factor)
Publisher Pergamon-Elsevier Science Ltd
Extent 376-381
Origin http://dx.doi.org/10.1016/j.marpolbul.2017.03.025
Access rights Closed access
Type Article
Web of Science ID WOS:000402217300056
URI https://repositorio.unifesp.br/handle/11600/54458

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