Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi

Show simple item record

dc.contributor.author Bonfim-Melo, Alexis [UNIFESP]
dc.contributor.author Ferreira, Eden R. [UNIFESP]
dc.contributor.author Mortara, Renato A. [UNIFESP]
dc.date.accessioned 2020-07-08T13:09:38Z
dc.date.available 2020-07-08T13:09:38Z
dc.date.issued 2018
dc.identifier http://dx.doi.org/10.3389/fmicb.2018.00360
dc.identifier.citation Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018.
dc.identifier.issn 1664-302X
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/54106
dc.description.abstract This study evaluated the participation of host cell Rho-family GTPases and their effector proteins in the actin-dependent invasion by Trypanosoma cruzi extracellular amastigotes (EAs). We observed that all proteins were recruited and colocalized with actin at EA invasion sites in live or fixed cells. EA internalization was inhibited in cells depleted in Rac1, N-WASP, and WAVE2. Time-lapse experiments with Rac1, N-WASP and WAVE2 depleted cells revealed that EA internalization kinetics is delayed even though no differences were observed in the proportion of EA-induced actin recruitment in these groups. Overexpression of constitutively active constructs of Rac1 and RhoA altered the morphology of actin recruitments to EA invasion sites. Additionally, EA internalization was increased in cells overexpressing CA-Rac1 but inhibited in cells overexpressing CA-RhoA. WT-Cdc42 expression increased EA internalization, but curiously, CA-Cdc42 inhibited it. Altogether, these results corroborate the hypothesis of EA internalization in non-phagocytic cells by a phagocytosis-like mechanism and present Rac1 as the key Rho-family GTPase in this process. en
dc.description.sponsorship Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.format.extent -
dc.language.iso eng
dc.publisher Frontiers Media Sa
dc.relation.ispartof Frontiers In Microbiology
dc.rights Acesso aberto
dc.subject Trypanosoma cruzi en
dc.subject extracellular amastigotes en
dc.subject Rho GTPases en
dc.subject actin en
dc.subject host cell invasion en
dc.title Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2012/21335-8, 2011/51475-3
dc.description.sponsorshipID CNPq: 302068/2016-3
dc.identifier.file WOS000426303300002.pdf
dc.identifier.doi 10.3389/fmicb.2018.00360
dc.description.source Web of Science
dc.identifier.wos WOS:000426303300002
dc.coverage Lausanne
dc.citation.volume 9



File

Name: WOS000426303300002.pdf
Size: 4.077Mb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account