Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies

Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies

Author Fernandes, K. R. Autor UNIFESP Google Scholar
Magri, A. M. P. Autor UNIFESP Google Scholar
Kido, H. W. Autor UNIFESP Google Scholar
Parisi, J. R. Google Scholar
Assis, L. Autor UNIFESP Google Scholar
Fernandes, K. P. S. Google Scholar
Mesquita-Ferrari, R. A. Google Scholar
Martins, V. C. Google Scholar
Plepis, A. M. Google Scholar
Zanotto, E. D. Google Scholar
Peitl, O. Google Scholar
Renno, A. C. M. Autor UNIFESP Google Scholar
Abstract The main purpose of the present work was to evaluate if low laser level therapy (LLLT) can improve the effects of Biosilicate (R)/PLGA (BS/PLGA) composites on cell viability and bone consolidation using a tibial defects of rats. The composites were characterized by scanning electron microscope (SEM) and reflection Fourier transform infrared spectrometer (FTIR). For the in vitro study, fibroblast and osteoblast cells were seeded in the extract of the composites irradiated or not with LLLT (Ga-Al-As, 808 nm, 10 J/cm(2)) to assess cell viability after 24, 48 and 72 h. For the in vivo study, 80 Wistar rats with tibial bone defects were distributed into 4 groups (BS

BS + LLLT

BS/PLGA and BS/PLGA + LLLT) and euthanized after 2 and 6 weeks. Laser irradiation Ga-Al-As (808 nm, 30 J/cm(2)) in the rats was performed 3 times a week. The SEM and FTIR results revealed that PLGA were successfully inserted into BS and the microparticles degraded over time. The in vitro findings demonstrated higher fibroblast viability in both BS/PLGA groups after 24 h and higher osteoblast viability in BS/PLGA + LLLT in all periods. As a conclusion, animals treated with BS/PLGA + LLLT demonstrated an improved material degradation and an increased amount of granulation tissue and newly formed bone.
Keywords Low level laser therapy
Biosilicate (R)
Bone repair
Cytotoxicity
Language English
Date 2017
Published in Journal Of Photochemistry And Photobiology B-Biology. Lausanne, v. 173, p. 258-265, 2017.
ISSN 1011-1344 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Sa
Extent 258-265
Origin http://dx.doi.org/10.1016/j.jphotobiol.2017.06.002
Access rights Closed access
Type Article
Web of Science ID WOS:000407984500026
URI http://repositorio.unifesp.br/handle/11600/51443

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