Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Autor Miyazaki, Celina Massumi Google Scholar
Pereira, Tamyris Paschoal Google Scholar
Tavares Mascagni, Daniela Branco Google Scholar
de Moraes, Marli Leite Autor UNIFESP Google Scholar
Ferreira, Marystela Google Scholar
Resumo In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (mu A cm(-2))/(mmol L-1) and a detection limit of 033 mmol L-1. (C) 2015 Elsevier B.V. All rights reserved.
Assunto Layer-by-layer
Monoamine oxidase-B
Dopamine detection
Liposomes
AmperometryAssembled Multilayer Films
Tandem Mass-Spectrometry
Ascorbic-Acid
Carbon Nanotubes
Uric-Acid
Electrochemical Sensors
Liquid-Chromatography
Glucose-Oxidase
Hemoglobin Immobilization
Amperometric Biosensors
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento FAPESP: 2014/15093-7
FAPESP: 2014/17519-1
Data 2016
Publicado em Materials Science & Engineering C-Materials For Biological Applications. Amsterdam, v. 58, p. 310-315, 2016.
ISSN 0928-4931 (Sherpa/Romeo, fator de impacto)
Editor Elsevier Science Bv
Extensão 310-315
Fonte http://dx.doi.org/10.1016/j.msec.2015.08.042
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000364247500036
URI http://repositorio.unifesp.br/handle/11600/49713

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