Phytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell death

Phytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell death

Title: Phytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell death;
Análise fitoquímica do extrato hidroetanólico de Turnera diffusa Willd e avaliação de seus efeitos na morte de astrócitos
Author Bezerra, Andreia Gomes Autor UNIFESP Google Scholar
Negri, Giuseppina Autor UNIFESP Google Scholar
Duarte-Almeida, Joaquim Mauricio Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Carlini, Elisaldo Araujo Autor UNIFESP Google Scholar
Abstract Objective: To evaluate the phytochemical composition of hydroethanolic extracts from powdered aerial parts of Turnera diffusa Willd (Turneraceae

T. diffusa), as well as its toxicity in astrocytes. Methods: Chemical analyses of hydroethanolic extract from powdered aerial parts of T. diffusa were carried out using HPLC-DAD-ESI-MS/MS. In vitro assays using astrocytes culture were performed to evaluate cell death. Results: Flavone-C, O-diglycosides, such as, luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, apigenin-8-C-[ 6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside and apigenin-7-O-6"-p-coumaroylglucoside were the main compounds found in this hydroethanolic extract. Concentration time-effect demonstrated the toxicity of this extract at a concentration of 1,000 mu g/mL in astrocyte culture, after 6 and 24 hours of incubation. Conclusion: In phytochemical analyses, important antioxidants (mainly flavonoids) were observed. T. diffusa extracts presented cytotoxic effect in high concentrations, leading to increased cell death in astrocyte culture.
Keywords Cognition/Drug Effects
Plant Extracts/Toxicity
Protective Agents/Toxicity
Antioxidants
Cell Death/Drug EffectsPerformance Liquid-Chromatography
Quadrupole Mass-Spectrometry
Ultraviolet Uv Radiation
In-Vitro Model
Phenolic-Compounds
Electrospray-Ionization
Antioxidant Properties
Acid-Derivatives
Oxidative Stress
Quality-Control
Language English
Sponsor Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)
Centro Brasileiro de Informacoes sobre Drogas Psicotropicas (CEBRID)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Date 2016
Published in Einstein-Sao Paulo. Sao paulo sp, v. 14, n. 1, p. 56-63, 2016.
ISSN 1679-4508 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 56-63
Origin http://dx.doi.org/10.1590/S1679-45082016AO3386
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000385715800012
SciELO ID S1679-45082016000100056 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/49379

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