Renovascular hypertension: effects of mesenchymal stem cells in the contralateral hypertensive kidney in rats

Renovascular hypertension: effects of mesenchymal stem cells in the contralateral hypertensive kidney in rats

Author de Oliveira-Sales, Elizabeth Barbosa Autor UNIFESP Google Scholar
Varela, Vanessa Araujo Autor UNIFESP Google Scholar
Maquigussa, Edgar Autor UNIFESP Google Scholar
Borges, Fernanda Teixeira Autor UNIFESP Google Scholar
Shimoura, Caroline Gusson Autor UNIFESP Google Scholar
Gomes, Guiomar Autor UNIFESP Google Scholar
Campos, Ruy Ribeiro Autor UNIFESP Google Scholar
Boim, Mirian Aparecida Autor UNIFESP Google Scholar
Abstract Mesenchymal stem cells (MSC) induced neovascularization and improved renal morphology of the stenotic kidney in 2 kidneys-1 clip (2K-1C) model of renovascular hypertension. The present study evaluated the effects of MSC in the contralateral hypertensive kidney. Three weeks after left renal artery occlusion, MSC were injected into the tail vein of the 2K-1C rats. Renal function and morphology were analyzed in both kidneys. Labeled MSC were found in stenotic and contralateral kidneys. Hypertensive 2K-1C animals presented increased circulating levels of Angiotensin II (Ang II) and renin. MSC prevented the progressive increase of blood pressure and reduced circulating Ang II and renin levels. Stenotic kidney showed reduced renal plasma flow (RPF) and glomerular filtration rate (GFR), whereas the contralateral kidney had a tendency (p > 0.5) of reduction in GFR in spite of unchanged RPF. MSC treatment caused an improvement in GFR with no effect of on RPF in the stenotic kidney. Contralateral kidney showed increased diuresis and natriuresis that were even higher in MSC-treated animals, indicating that cell treatment improved the capacity of the contralateral kidney to excrete sodium. Contralateral kidney expressed higher levels of inflammatory cytokines (IL-6, TNF-) and signs of fibrosis, which were attenuated by MSC treatment. MSC treatment improved the stenotic kidney function, and it was also beneficial to the contralateral hypertensive kidney because it improved the morphology and preserved its capacity to excrete sodium.
Keywords Mesenchymal Stem Cells
Renovascular Hypertension
Renal Function
Renin-Angiotensin SystemRenal-Artery Stenosis
Endothelial Progenitor Cells
Goldblatt Hypertension
Oxidative Stress
Language English
Sponsor Coordenacao de Aperfeicoamento de Nivel Superior (CAPES)
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Date 2016
Published in Clinical And Experimental Hypertension. Philadelphia, v. 38, n. 7, p. 586-593, 2016.
ISSN 1064-1963 (Sherpa/Romeo, impact factor)
Publisher Hospital Clinicas, Univ Sao Paulo
Extent 586-593
Access rights Closed access
Type Article
Web of Science ID WOS:000386114700004

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