Characterization of paracoccidioides brasiliensis by ft-ir spectroscopy and nanotechnology

Characterization of paracoccidioides brasiliensis by ft-ir spectroscopy and nanotechnology

Author Ferreira, Isabelle Google Scholar
Ferreira-Strixino, Juliana Google Scholar
Castilho, Maiara L. Google Scholar
Campos, Claudia Barbosa Ladeira de Autor UNIFESP Google Scholar
Tellez, Claudio Google Scholar
Raniero, Leandro Google Scholar
Abstract Paracoccidioides brasiliensis, the etiological agent of paracoccidioidomycosis, is a dimorphic fungus existing as mycelia in the environment (or at 25 degrees C in vitro) and as yeast cells in the human host (or at 37 degrees C in vitro). Because mycological examination of lesions in patients frequently is unable to show the presence of the fungus and serological tests can misdiagnose the disease with other mycosis, the development of new approach's for molecular identification of P. brasiliensis spurges is needed. This study describes the use of a gold nanoprobe of a known gene sequence of P. brasiliensis as a molecular tool to identify P. brasiliensis by regular polymerase chain reaction (PCR) associated with a colorimetric methods. This approach is suitable for testing in remote areas because it does not require any further step than gene amplification, being safer and cheaper than electrophoresis methods. The proposed test showed a color change of the PCR reaction mixture from red to blue in negative samples, whereas the solution remains red in positive samples. We also performed a Fourier Transform Infrared (FT-IR) Spectroscopy analysis to characterize and compare the chemical composition between yeast and mycelia forms, which revealed biochemical differences between these two forms. The analysis of the spectra showed that differences were distributed in chemical bonds of proteins, lipids and carbohydrates. The most prominent difference between both forms was vibration modes related to 1,3-beta-glucan usually found in mycelia and 1,3-alpha-glucan found in yeasts and also chitin forms. In this work, we introduce FT-IR as a new method suitable to reveal overall differences that biochemically distinguish each form of P. brasiliensis that could be additionally used to discriminate biochemical differences among a single form under distinct environmental conditions. (C) 2015 Elsevier B.V. All rights reserved.
Keywords Paracoccidioides Brasiliensis
Yeast
Mycelia
Gold Nanoprobes
Ft-IrGold Nanoparticles
Cell-Wall
Glycoprotein Gp43
Gene-Expression
Dna
Chitin
Yeast
Identification
Antigen
Protein
Language English
Sponsor FAPESP [2009/09559-5]
CNPq [301066/2009-4, 301022/2012-7]
Grant number FAPESP: 2009/09559-5
CNPq: 301066/2009-4
CNPq:301022/2012-7
Date 2016
Published in Spectrochimica Acta Part A-Molecular And Biomolecular Spectroscopy. Oxford, v. 152, p. 397-403, 2016.
ISSN 1386-1425 (Sherpa/Romeo, impact factor)
Publisher Univ Brasilia
Extent 397-403
Origin https://doi.org/10.1016/j.saa.2015.07.061
Access rights Closed access
Type Article
Web of Science ID WOS:000365367100049
URI http://repositorio.unifesp.br/handle/11600/49195

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account