Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura

Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura

Alternative title Effects of Physical Exercise in the Ampk alpha Expression and Activity in High-fat Diet Induced Obese Rats
Author Pauli, Jose Rodrigo Autor UNIFESP Google Scholar
Ropelle, Eduardo Rochete Google Scholar
Cintra, Dennys Esper Google Scholar
Souza, Claudio Teodoro de Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Introduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved.
Keywords obesity
insulin resistance
physical education and training
Language Portuguese
Date 2009-03-01
Published in Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009.
ISSN 1517-8692 (Sherpa/Romeo, impact factor)
Publisher Soc Brasileira Med Esporte
Extent 98-103
Origin http://dx.doi.org/10.1590/S1517-86922009000200003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000266133400003
SciELO ID S1517-86922009000200003 (statistics in SciELO)
URI http://repositorio.unifesp.br/11600/45256

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