Glycomic Analysis of Tear and Saliva in Ocular Rosacea Patients: The Search for a Biomarker

Glycomic Analysis of Tear and Saliva in Ocular Rosacea Patients: The Search for a Biomarker

Author Vieira, Ana Carolina Autor UNIFESP Google Scholar
An, Hyun Joo Google Scholar
Ozcan, Sureyya Google Scholar
Kim, Jae-Han Google Scholar
Lebrilla, Carlito B. Google Scholar
Mannis, Mark J. Google Scholar
Institution Univ Calif Davis
Universidade Federal de São Paulo (UNIFESP)
Abstract The purpose of this study was to study changes in glycosylation in tear and saliva obtained from control and ocular rosacea patients in order to identify potential biomarkers for rosacea. Tear fluid was collected from 51 subjects (28 healthy controls and 23 patients with ocular rosacea). Saliva was collected from 42 of the same subjects (25 controls and 17 patients). Pooled and individual samples were examined to determine overall glycan profiles and individual variations in glycosylation. O-and N- glycans were released from both patients and control subjects. Released glycans were purified and enriched by solid-phase extraction (SPE) with graphitized carbon. Glycans were eluted based on glycan size and polarity. SPE fractions were then analyzed by high-resolution mass spectrometry. Glycan compositions were assigned by accurate masses. Their structures were further elucidated by tandem mass spectrometric using collision-induced dissociation (CID), and specific linkage information was obtained by exoglycosidase digestion. N- and O-glycans were released from 20-mu L samples without protein identification, separation, and purification. Approximately 50 N-glycans and 70 O-glycans were globally profiled by mass spectrometry. Most N-glycans were highly fucosylated, while 0-glycans were sulfated. Normal tear fluid and saliva contain highly fucosylated glycans. The numbers of sulfated glycans were dramatically increased in tear and saliva of rosacea patients compared to controls. Glycans found in tear and saliva from roseatic patients present highly quantitative similarity. The abundance of highly fucosylated N-glycans in the control samples and sulfated O-glycans in ocular rosacea patient samples may lead to the discovery of an objective diagnostic marker for the disease.
Keywords biomarkers
fucosylation
glycans
glycomic analysis
mucin
ocular rosacea
oligosaccharides
tear
saliva
Language English
Sponsor Research to Prevent Blindness, Inc., New York, NY
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-07-01
Published in Ocular Surface. New York: Ethis Communicatins, Inc, v. 10, n. 3, p. 184-192, 2012.
ISSN 1542-0124 (Sherpa/Romeo, impact factor)
Publisher Ethis Communicatins, Inc
Extent 184-192
Origin http://dx.doi.org/10.1016/j.jtos.2012.04.003
Access rights Closed access
Type Article
Web of Science ID WOS:000307262200006
URI http://repositorio.unifesp.br/11600/45059

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