Immunohistochemical analysis of adhesion molecule expression on muscle biopsy specimens from patients with juvenile dermatomyositis

Immunohistochemical analysis of adhesion molecule expression on muscle biopsy specimens from patients with juvenile dermatomyositis

Author Sallum, Adriana ME Google Scholar
Marie, Suely KN Google Scholar
Wakamatsu, Alda Google Scholar
Sachetti, Silvana Google Scholar
Vianna, Maria AG Google Scholar
Silva, Clóvis AA Google Scholar
Kiss, Maria HB Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Adolfo Lutz Inst
Abstract Objective. To assess expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on muscle biopsy specimens from patients with untreated juvenile dermatomyositis (JDM).Methods. Histochemical and immunohistochemical tests for ICAM-1 and VCAM-1 were performed on serial frozen sections from 27 JDM muscle biopsy specimens. ICAM-1 and VCAM-1 expression was analyzed on capillaries, perimysial and endomysial large vessels, and muscle fibers. Expression was assessed and graded semiquantitatively.Results. Increased ICAM-1 expression was observed on capillaries and perimysial large vessels on semiquantitative analysis, and was statistically more evident than on endomysial large vessels. In all cases, only a few muscle vessels showed expression of VCAM-1. Expression of ICAM-1 and VCAM-1 was observed on few muscle fibers.Conclusion. The observation of ICAM-1 expression on muscle vessels, mainly on capillaries of patients with untreated JDM compared to controls, and. VCAM-1 expression to a lesser extent, mostly on muscle vessels surrounded by inflammatory infiltrate, supports the participation of these adhesion molecules in the pathologic mechanism of vascular injury in JDM.
Keywords juvenile dermatomyositis
adhesion molecules
muscle biopsy
intercellular adhesion molecule-1
vascular cell adhesion molecule-1
Language English
Date 2004-04-01
Published in Journal Of Rheumatology. Toronto: J Rheumatol Publ Co, v. 31, n. 4, p. 801-807, 2004.
ISSN 0315-162X (Sherpa/Romeo, impact factor)
Publisher J Rheumatol Publ Co
Extent 801-807
Origin http://www.jrheum.org/content/31/4/801
Access rights Closed access
Type Article
Web of Science ID WOS:000220673000031
URI http://repositorio.unifesp.br/11600/44674

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