Bioengineered teeth from cultured rat tooth bud cells

Bioengineered teeth from cultured rat tooth bud cells

Author Duailibi, M. T. Google Scholar
Duailibi, S. E. Google Scholar
Young, C. S. Google Scholar
Bartlett, J. D. Google Scholar
Vacanti, J. P. Google Scholar
Yelick, P. C. Google Scholar
Institution Forsyth Inst
Harvard Univ
Universidade Federal de São Paulo (UNIFESP)
Massachusetts Gen Hosp
Abstract The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
Keywords tooth tissue engineering
dental stem cells
Language English
Date 2004-07-01
Published in Journal Of Dental Research. Alexandria: Int Amer Assoc Dental Researchi A D R/a A D R, v. 83, n. 7, p. 523-528, 2004.
ISSN 0022-0345 (Sherpa/Romeo, impact factor)
Publisher Int Amer Assoc Dental Researchi A D R/a A D R
Extent 523-528
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000226598200003

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