C-kit expression in human osteosarcoma and in vitro assays

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dc.contributor.author Miiji, Luciana Nakao Odashiro [UNIFESP]
dc.contributor.author Petrilli, Antonio Sergio [UNIFESP]
dc.contributor.author Di Cesare, Sebastian
dc.contributor.author Odashiro, Alexandre Nakao [UNIFESP]
dc.contributor.author Burnier, Miguel Noel Nascente [UNIFESP]
dc.contributor.author Toledo, Silvia Regina Caminada de [UNIFESP]
dc.contributor.author Garcia, Reynaldo Jesus
dc.contributor.author Alves, Maria Teresa de Seixas [UNIFESP]
dc.date.accessioned 2018-06-15T17:44:18Z
dc.date.available 2018-06-15T17:44:18Z
dc.date.issued 2011-01-01
dc.identifier http://www.ijcep.com/1109006A.html
dc.identifier.citation International Journal Of Clinical And Experimental Pathology. Madison: E-century Publishing Corp, v. 4, n. 8, p. 775-781, 2011.
dc.identifier.issn 1936-2625
dc.identifier.uri http://repositorio.unifesp.br/11600/44032
dc.description.abstract Biologic agents targeting oncogenes have encourage researchs trying to correlate the role of tyrosine kinase in the pathogenesis of tumours. Osteosarcoma is a high grade aggressive neoplasm with poor survival. Our aim was to investigate c-kit immunoexpression, its prognostic relevance for patients with osteosarcoma, and the effect of imatinib mesylate (STI571) on proliferation and invasion of the human osteosarcoma cell line. A retrospective immunohistochemical study was performed on archival formalin-fixed paraffin-embedded specimens from 52 patients with high-grade primary osteosarcoma of extremities treated at the Pediatric Oncology Institute (IOP, GRAAC) and archived in the Department of Pathology, Federal University of Sao Paulo. Only pre-chemotherapy specimens were analyzed. Strongly stained cytoplasm and membrane cells were taken as positive. Human osteosarcoma cells from line MG-63 were incubated and the inhibitory effect of imatinib mesylate (STI571) on cell proliferation and invasion was studied. In 24 cases (46.15%), c-kit was expressed by the cells and c-kit-positive tumors exhibited lower necrosis post-chemotherapy. No correlation was found between c-kit expression and overall and disease-free survival. Imatinib mesylate decreased the rates of cell growth of osteosarcoma cells in low doses and invasion in high doses C-kit-positive tumors had worse response to chemotherapy and imatinib mesylate can play a role in blocking or decreasing the rate of growth of osteosarcoma cells, but not the invasive capacity of these neoplastic cells. These data suggested that imatinib mesylate could be a therapeutic target of strategies against osteosarcoma tumors. Further studies are necessary to confirm this indication. en
dc.format.extent 775-781
dc.language.iso eng
dc.publisher E-century Publishing Corp
dc.relation.ispartof International Journal Of Clinical And Experimental Pathology
dc.rights Acesso aberto
dc.subject Osteosarcoma en
dc.subject c-kit en
dc.subject immunohistochemistry en
dc.subject in vitro assays en
dc.subject prognosis en
dc.title C-kit expression in human osteosarcoma and in vitro assays en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution McGill Univ
dc.contributor.institution Ctr Hosp Afillie Univ Quebec
dc.description.affiliation Univ Fed Sao Paulo, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, IOP, GRAACC, UNIFESP,Dept Pediat, Sao Paulo, Brazil
dc.description.affiliation McGill Univ, Henry C Witelson Ocular Pathol Lab, Montreal, PQ, Canada
dc.description.affiliation Ctr Hosp Afillie Univ Quebec, Dept Pathol, Quebec City, PQ, Canada
dc.description.affiliation Univ Fed Sao Paulo, Dept Orthoped Surg, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, IOP, GRAACC, UNIFESP,Dept Pediat, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Orthoped Surg, Sao Paulo, Brazil
dc.identifier.file WOS000298346300006.pdf
dc.description.source Web of Science
dc.identifier.wos WOS:000298346300006



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