Author |
Miiji, Luciana Nakao Odashiro
![]() ![]() Petrilli, Antonio Sergio ![]() ![]() Di Cesare, Sebastian ![]() Odashiro, Alexandre Nakao ![]() ![]() Burnier, Miguel Noel Nascente ![]() ![]() Toledo, Silvia Regina Caminada de ![]() ![]() Garcia, Reynaldo Jesus ![]() Alves, Maria Teresa de Seixas ![]() ![]() |
Institution | Universidade Federal de São Paulo (UNIFESP) McGill Univ Ctr Hosp Afillie Univ Quebec |
Abstract | Biologic agents targeting oncogenes have encourage researchs trying to correlate the role of tyrosine kinase in the pathogenesis of tumours. Osteosarcoma is a high grade aggressive neoplasm with poor survival. Our aim was to investigate c-kit immunoexpression, its prognostic relevance for patients with osteosarcoma, and the effect of imatinib mesylate (STI571) on proliferation and invasion of the human osteosarcoma cell line. A retrospective immunohistochemical study was performed on archival formalin-fixed paraffin-embedded specimens from 52 patients with high-grade primary osteosarcoma of extremities treated at the Pediatric Oncology Institute (IOP, GRAAC) and archived in the Department of Pathology, Federal University of Sao Paulo. Only pre-chemotherapy specimens were analyzed. Strongly stained cytoplasm and membrane cells were taken as positive. Human osteosarcoma cells from line MG-63 were incubated and the inhibitory effect of imatinib mesylate (STI571) on cell proliferation and invasion was studied. In 24 cases (46.15%), c-kit was expressed by the cells and c-kit-positive tumors exhibited lower necrosis post-chemotherapy. No correlation was found between c-kit expression and overall and disease-free survival. Imatinib mesylate decreased the rates of cell growth of osteosarcoma cells in low doses and invasion in high doses C-kit-positive tumors had worse response to chemotherapy and imatinib mesylate can play a role in blocking or decreasing the rate of growth of osteosarcoma cells, but not the invasive capacity of these neoplastic cells. These data suggested that imatinib mesylate could be a therapeutic target of strategies against osteosarcoma tumors. Further studies are necessary to confirm this indication. |
Keywords |
Osteosarcoma
c-kit immunohistochemistry in vitro assays prognosis |
Language | English |
Date | 2011-01-01 |
Published in | International Journal Of Clinical And Experimental Pathology. Madison: E-century Publishing Corp, v. 4, n. 8, p. 775-781, 2011. |
ISSN | 1936-2625 (Sherpa/Romeo, impact factor) |
Publisher | E-century Publishing Corp |
Extent | 775-781 |
Origin |
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Access rights | Open access ![]() |
Type | Article |
Web of Science ID | WOS:000298346300006 |
URI | http://repositorio.unifesp.br/11600/44032 |
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