Ultrastructural analysis of the hDSC interactions with biodegradable 3D scaffolds

Ultrastructural analysis of the hDSC interactions with biodegradable 3D scaffolds

Author Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Ferreira, L. M. Autor UNIFESP Google Scholar
Tanaka, F. A. O. Google Scholar
Vacanti, J. P. Google Scholar
Yelick, P. C. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract This study observed the behavior of hDSCs on 3 biodegradable 3D scaffolds surfaces coated with collagen: PGA; PHB; Silk and PCL. Based on the demonstrated ability for scaffold surfaces to dramatically influence cell viability and adhesion, in this study we characterize hDSC viability, affinity, and differentiation on 3D scaffolds. Isolated human tooth cells were obtained from patients scheduled extraction (CONEP-13083) and plated and expanded in standard tissue culture conditions. After 20 and 30 days, hDSCs were observed under inverted microscopy, followed by TEM and SEM analyses, which revealed the activity of hDSCs on each scaffold type, including cells adhesion on the biomaterial surface. The high cells activity was observed where we found more mitochondria and rugous endoplasmatic reticulum representing high energy and mRNA production for cells proliferation in similarity with the control group. The PGA and PHB exhibited the better hDSCs adhesion with 3D scaffold interface.
Language English
Date 2012-01-01
Published in Innovative Developments On Virtual And Physical Prototyping. Boca Raton: Crc Press-taylor & Francis Group, p. 25-28, 2012.
Publisher Crc Press-taylor & Francis Group
Extent 25-28
Origin https://doi.org/10.1201/b11341-6
Access rights Closed access
Type Conference paper
Web of Science ID WOS:000305445600003
URI http://repositorio.unifesp.br/11600/43978

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