Aberrant phenotypes in acute myeloid leukemia: a high frequency and clinical significance

Aberrant phenotypes in acute myeloid leukemia: a high frequency and clinical significance

Author Bahia, Daniella Marcia Maranhão Autor UNIFESP Google Scholar
Yamamoto, Mihoko Autor UNIFESP Google Scholar
Chauffaille, Maria de Lourdes Lopes Ferrari Autor UNIFESP Google Scholar
Kimura, Eliza Yuriko Sugano Autor UNIFESP Google Scholar
Bordin, Jose Orlando Autor UNIFESP Google Scholar
Filgueiras, MAC Google Scholar
Kerbauy, José Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background and Objectives. Immunophenotyping is an essential method for diagnosis and classification of acute myeloid leukemias (AML), and its extensive use could identify blast cell subpopulations with aberrant phenotypes rarely seen in normal myelopoiesis. The aberrant phenotypes have been correlated with clinical, morphologic and prognostic features but their occurrence in AML differs in the various studies.Design and Methods. In this study we analyzed 35 cases of AML, examining them for aberrant phenotypes by multiparametric flow cytometry. Co-expression of lymphoid-associated markers in myeloblasts and asynchronous antigen expression were correlated with clinical features.Results. Aberrant phenotypes were found in 88.6% of the cases studied. In this group, cross-lineage antigen expression was present in 34.3% and asynchronous expression in 82.4% of the cases. CD7 was the most frequent lymphoid-associated antigen. Among the cases of asynchronous antigen expression, the most frequent phenotype was CD117(+) and/or CD34(+) in association with CD11c(+), followed by CD15(+) and CD65(+), corresponding to 67.6%, 61.7% and 50.0% of the cases, respectively. Twenty out of 33 patients were available for complete remission assessment. The CD117+ CD15+ phenotype correlated significantly with complete remission achievement and with the lack of unfavorable chromosome associations.Interpretation and Conclusions. We conclude that aberrant phenotypes, as they are described here, are present in the great majority of cases of AML, asynchronous antigen expression being the most frequent example, and that CD117+ CD15+ phenotype shows a relevant association with clinical prognosis. (C) 2001, Ferrata Storti Foundation.
Keywords aberrant phenotype
flow cytometry
AML
prognosis
Language English
Date 2001-08-01
Published in Haematologica. Pavia: Ferrata Storti Foundation, v. 86, n. 8, p. 801-806, 2001.
ISSN 0390-6078 (Sherpa/Romeo, impact factor)
Publisher Ferrata Storti Foundation
Extent 801-806
Origin http://www.haematologica.org/content/86/8/801
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000170865100003
URI http://repositorio.unifesp.br/11600/43736

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