GP43 from Paracoccidioides brasiliensis inhibits macrophage functions. An evasion mechanism of the fungus

GP43 from Paracoccidioides brasiliensis inhibits macrophage functions. An evasion mechanism of the fungus

Author Popi, Ana Flavia Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Macrophages constitute one of the primary cellular mechanisms that impairs parasite invasion of host tissues. The phagocytic and microbicidal properties of these cells can be modulated by specific membrane receptors involved in cell-microorganism interactions. Gp43, the main antigen secreted by Paracoccidiodes brasiliensis (Pb), the causative agent of Paracoccidioidomycosis, is a high mannose glycoprotein. The role played by gp43 in the pathogenesis of the disease is not completely known. Here, we describe the influence of this molecule on the interaction between peritoneal murine macrophages and Pb. Phagocytosis of Pb, live or heat-killed, by adherent peritoneal cells from both, B10.A (susceptible) and A/Sn (resistant) mice, was evaluated. Addition of different concentrations of gp43 to the culture medium inhibited, in a dose-dependent pattern, phagocytosis of live or heat-killed Pb by peritoneal macrophages from both B10.A and A/Sn mice. Gp43 also inhibits phagocytosis of zymosan particles but did not interfere with the uptake of opsonized sheep red blood cells. It was also shown that both gp43 and heat-killed Pb have an inhibitory effect on the release of NO by zymosan stimulated macrophages. Finally, we demonstrated that gp43 inhibits the fungicidal ability of macrophages from both lineages. Based on these data, it is suggested that gp43 can be considered one of the evasion mechanisms for the installation of primary infection in susceptible hosts. (C) 2002 Elsevier Science (USA). All rights reserved.
Keywords Paracoccidiodes brasiliensis
microbicidal activity
escape mechanism
Language English
Date 2002-07-01
Published in Cellular Immunology. San Diego: Academic Press Inc Elsevier Science, v. 218, n. 1-2, p. 87-94, 2002.
ISSN 0008-8749 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 87-94
Access rights Closed access
Type Article
Web of Science ID WOS:000179962300009

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