DNA and bone structure preservation in medieval human skeletons

DNA and bone structure preservation in medieval human skeletons

Author Coulson-Thomas, Yvette May Autor UNIFESP Google Scholar
Norton, Andrew L. Google Scholar
Coulson-Thomas, Vivien Jane Autor UNIFESP Google Scholar
Florencio-Silva, Rinaldo Autor UNIFESP Google Scholar
Ali, Nadir Google Scholar
Elmrghni, Samir Google Scholar
Gil, Cristiane D. Autor UNIFESP Google Scholar
Sasso, Gisela R. S. Autor UNIFESP Google Scholar
Dixon, Ronald A. Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Lincoln Univ
Univ Durham
Univ Cambridge
Abstract Morphological and ultrastructural data from archaeological human bones are scarce, particularly data that have been correlated with information on the preservation of molecules such as DNA. Here we examine the bone structure of macroscopically well-preserved medieval human skeletons by transmission electron microscopy and immunohistochemistry, and the quantity and quality of DNA extracted from these skeletons. DNA technology has been increasingly used for analyzing physical evidence in archaeological forensics; however, the isolation of ancient DNA is difficult since it is highly degraded, extraction yields are low and the co-extraction of PCR inhibitors is a problem. We adapted and optimised a method that is frequently used for isolating DNA from modern samples, Chelex (R) 100 (BioRad) extraction, for isolating DNA from archaeological human bones and teeth. the isolated DNA was analysed by real-time PCR using primers targeting the sex determining region on the Y chromosome (SRY) and STR typing using the AmpFlSTR (R) Identifiler PCR Amplification kit. Our results clearly show the preservation of bone matrix in medieval bones and the presence of intact osteocytes with well preserved encapsulated nuclei. in addition, we show how effective Chelex1 100 is for isolating ancient DNA from archaeological bones and teeth. This optimised method is suitable for STR typing using kits aimed specifically at degraded and difficult DNA templates since amplicons of up to 250 bp were successfully amplified. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Keywords Ancient DNA
Archaeological bone
Archaeological teeth
Chelex
DNA typing
Electron microscopy
Language English
Date 2015-06-01
Published in Forensic Science International. Clare: Elsevier B.V., v. 251, p. 186-194, 2015.
ISSN 0379-0738 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 186-194
Origin http://dx.doi.org/10.1016/j.forsciint.2015.04.005
Access rights Closed access
Type Article
Web of Science ID WOS:000354125900028
URI http://repositorio.unifesp.br/handle/11600/39126

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