Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology

Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology

Autor Mendes, N. J. Google Scholar
Cruz, V. P. Google Scholar
Mendonca, Fernando Fernandes Autor UNIFESP Google Scholar
Pardo, B. G. Google Scholar
Coelho, R. Google Scholar
Ashikaga, F. Y. Google Scholar
Camargo, S. M. Autor UNIFESP Google Scholar
Martinez, P. Google Scholar
Oliveira, C. Google Scholar
Santos, M. N. Google Scholar
Foresti, F. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Santiago de Compostela
IPMA IP
Univ Algarve
Resumo Among the pelagic shark species most impacted by fisheries, the oceanic whitetip shark (Carcharhinus longimanus) has shown signs of population declines. in this study, we developed 12 microsatellite markers using next generation sequencing (454 pyrosequencing) in C. longimanus for further use in population screening. the genetic diversity of these loci was assessed in 28 individuals and a total of 35 alleles were identified, ranging from four to eight alleles per locus (5.9 alleles on average). Cross-species amplification of these markers was successful in five loci for C. acronotus, C. perezi and Galeocerdo cuvier. the developed microsatellite markers will be useful in analyzing the population genetics structure of the oceanic whitetip shark and other Carcharhinidae species.
Palavra-chave Conservation
Population genetics
Next generation sequencing
Carcharhinus
Idioma Inglês
Financiador FCT
Portuguese Foundation for Science and Technology
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento Portuguese Foundation for Science and Technology: SFRH/BPD/93936/2013
FAPESP: 2013/14555-4
FAPESP: BIOTA 2011/23787-0
FAPESP: 2010/51903-2
Data de publicação 2015-06-01
Publicado em Conservation Genetics Resources. Dordrecht: Springer, v. 7, n. 2, p. 585-589, 2015.
ISSN 1877-7252 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 585-589
Fonte http://dx.doi.org/10.1007/s12686-015-0435-5
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000354213300069
Endereço permanente http://repositorio.unifesp.br/handle/11600/39118

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta