Aerobic Exercise Training Prevents the Onset of Endothelial Dysfunction via Increased Nitric Oxide Bioavailability and Reduced Reactive Oxygen Species in an Experimental Model of Menopause

Aerobic Exercise Training Prevents the Onset of Endothelial Dysfunction via Increased Nitric Oxide Bioavailability and Reduced Reactive Oxygen Species in an Experimental Model of Menopause

Author Braga, Viviane Aparecida Vieira Nogueira Autor UNIFESP Google Scholar
Couto, Gisele Kruger Google Scholar
Lazzarin, Mariana Cruz Autor UNIFESP Google Scholar
Rossoni, Luciana Venturini Google Scholar
Medeiros, Alessandra Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract ObjectivePrevious studies have shown that estrogen deficiency, arising in postmenopause, promotes endothelial dysfunction. This study evaluated the effects of aerobic exercise training on endothelial dependent vasodilation of aorta in ovariectomized rats, specifically investigating the role of nitric oxide (NO) and reactive oxygen species (ROS).MethodsFemale Wistar rats ovariectomized (OVX - n= 20) or with intact ovary (SHAM - n= 20) remained sedentary (OVX and SHAM) or performed aerobic exercise training on a treadmill 5 times a week for a period of 8 weeks (OVX-TRA and SHAM-TRA). in the thoracic aorta the endothelium-dependent and - independent vasodilation was assessed by acetylcholine (ACh) and sodium nitroprusside (SNP), respectively. Certain aortic rings were incubated with L-NAME to assess the NO modulation on the ACh-induced vasodilation. the fluorescence to dihydroethidium in aortic slices and plasma nitrite/nitrate concentrations were measured to evaluate ROS and NO bioavailability, respectively.ResultsACh-induced vasodilation was reduced in OVX rats as compared SHAM (Rmax: SHAM: 86 +/-3.3 vs. OVX: 57+/-3.0%, p<0.01). Training prevented this response in OVX-TRA (Rmax: OVX-TRA: 88+/-2.0%, p<0.01), while did not change it in SHAM-TRA (Rmax: SHAM-TRA: 80+/-2.2%, p<0.01). the L-NAME incubation abolished the differences in ACh-induced relaxation among groups. SNP-induced vasodilation was not different among groups. OVX reduced nitrite/nitrate plasma concentrations and increased ROS in aortic slices, training as effective to restore these parameters to the SHAM levels.ConclusionsExercise training, even in estrogen deficiency conditions, is able to improve endothelial dependent vasodilation in rat aorta via enhanced NO bioavailability and reduced ROS levels.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2012/17709-0
FAPESP: 2010/50048-1
Date 2015-04-29
Published in Plos One. San Francisco: Public Library Science, v. 10, n. 4, 13 p., 2015.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 13
Origin http://dx.doi.org/10.1371/journal.pone.0125388
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000353711600146
URI http://repositorio.unifesp.br/handle/11600/39015

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