Pinealectomy interferes with the circadian clock genes expression in white adipose tissue

Pinealectomy interferes with the circadian clock genes expression in white adipose tissue

Author Mendes de Farias, Talita da Silva Google Scholar
Oliveira, Ariclecio Cunha de Google Scholar
Andreotti, Sandra Google Scholar
Amaral, Fernanda Gaspar do Autor UNIFESP Google Scholar
Chimin, Patricia Google Scholar
Alves de Proenca, Andre Ricardo Google Scholar
Leonardo Torres Leal, Francisco Google Scholar
Laurato Sertie, Rogerio Antonio Google Scholar
Campana, Amanda Baron Google Scholar
Lopes, Andressa Bolsoni Google Scholar
Souza, Arnaldo Henrique de Google Scholar
Cipolla-Neto, Jose Google Scholar
Lima, Fabio Bessa Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Melatonin, the main hormone produced by the pineal gland, is secreted in a circadian manner (24-hr period), and its oscillation influences several circadian biological rhythms, such as the regulation of clock genes expression (chronobiotic effect) and the modulation of several endocrine functions in peripheral tissues. Assuming that the circadian synchronization of clock genes can play a role in the regulation of energy metabolism and it is influenced by melatonin, our study was designed to assess possible alterations as a consequence of melatonin absence on the circadian expression of clock genes in the epididymal adipose tissue of male Wistar rats and the possible metabolic repercussions to this tissue. Our data show that pinealectomy indeed has impacts on molecular events: it abolishes the daily pattern of the expression of Clock, Per2, and Cry1 clock genes and Ppar expression, significantly increases the amplitude of daily expression of Rev-erb, and affects the pattern of and impairs adipokine production, leading to a decrease in leptin levels. However, regarding some metabolic aspects of adipocyte functions, such as its ability to synthesize triacylglycerols from glucose along 24hr, was not compromised by pinealectomy, although the daily profile of the lipogenic enzymes expression (ATP-citrate lyase, malic enzyme, fatty acid synthase, and glucose-6-phosphate dehydrogenase) was abolished in pinealectomized animals.
Keywords adipose tissue
clock genes
melatonin
metabolism
pinealectomy
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2010/20719-1
Date 2015-04-01
Published in Journal of Pineal Research. Hoboken: Wiley-Blackwell, v. 58, n. 3, p. 251-261, 2015.
ISSN 0742-3098 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 251-261
Origin http://dx.doi.org/10.1111/jpi.12211
Access rights Closed access
Type Article
Web of Science ID WOS:000351360400001
URI http://repositorio.unifesp.br/handle/11600/38978

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