Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats

Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats

Author Soares de Andrade, Luiz Henrique Autor UNIFESP Google Scholar
Almeida Monteiro de Moraes, Wilson Max Autor UNIFESP Google Scholar
Matsuo Junior, Eduardo Hiroshi Autor UNIFESP Google Scholar
Carvalho de Moura, Elizabeth de Orleans Autor UNIFESP Google Scholar
Moreira Antunes, Hanna Karen Autor UNIFESP Google Scholar
Montemor, Jairo Autor UNIFESP Google Scholar
Antonio, Ednei Luiz Autor UNIFESP Google Scholar
Bocalini, Danilo Sales Autor UNIFESP Google Scholar
Serra, Andrey Jorge Autor UNIFESP Google Scholar
Ferreira Tucci, Paulo Jose Autor UNIFESP Google Scholar
Brum, Patricia Chakur Google Scholar
Medeiros, Alessandra Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Sao Judas Tadeu
Univ Nove Julho
Universidade de São Paulo (USP)
Abstract The activity of the ubiquitin proteasome system (UPS) and the level of oxidative stress contribute to the transition from compensated cardiac hypertrophy to heart failure in hypertension. Moreover, aerobic exercise training (AET) is an important therapy for the treatment of hypertension, but its effects on the UPS are not completely known. the aim of this study was to evaluate the effect of AET on UPS's activity and oxidative stress level in heart of spontaneously hypertensive rats (SHR). A total of 53 Wi-star and SHR rats were randomly divided into sedentary and trained groups. the AET protocol was 59/week in treadmill for 13 weeks. Exercise tolerance test, non-invasive blood pressure measurement, echocardiographic analyses, and left ventricle hemodynamics were performed during experimental period. the expression of ubiquitinated proteins, 4-hydroxynonenal (4-HNE), Akt, phospho-Akt(ser473), GSK3 beta, and phospho-GSK3 beta(ser9) were analyzed by western blotting. the evaluation of lipid hydroperoxide concentration was performed using the xylenol orange method, and the proteasomal chymotrypsin-like activity was measured by fluorimetric assay. Sedentary hypertensive group presented cardiac hypertrophy, unaltered expression of total Akt, phospho-Akt, total GSK3 beta and phospho-GSK3 beta, UPS hyperactivity, increased lipid hydroperoxidation as well as elevated expression of 4-HNE but normal cardiac function. in contrast, AET significantly increased exercise tolerance, decreased resting systolic blood pressure and heart rate in hypertensive animals. in addition, the AET increased phospho-Akt expression, decreased phospho-GSK3 beta, and did not alter the expression of total Akt, total GSK3 beta, and ubiquitinated proteins, however, significantly attenuated 4-HNE levels, lipid hydroperoxidation, and UPS's activity toward normotensive group levels. Our results provide evidence for the main effect of AET on attenuating cardiac ubiquitin proteasome hyperactivity and oxidative stress in SHR rats.
Keywords Hypertension
Aerobic exercise training
Cardiac remodeling
Ubiquitin proteasome system
Oxidative stress
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CNPq: 474085/2011-2
Date 2015-04-01
Published in Molecular and Cellular Biochemistry. Dordrecht: Springer, v. 402, n. 1-2, p. 193-202, 2015.
ISSN 0300-8177 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 193-202
Access rights Closed access
Type Article
Web of Science ID WOS:000352849800019

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