Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints

Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints

Author Moreira, Juliana Junqueira Autor UNIFESP Google Scholar
Moraes, Ana Paula Lopes de Autor UNIFESP Google Scholar
Brossi, Patricia Monaco Autor UNIFESP Google Scholar
Machado, Thais Sodré de Lima Autor UNIFESP Google Scholar
Michelacci, Yara Maria Autor UNIFESP Google Scholar
Massoco, Cristina de Oliveira Google Scholar
Baccarin, Raquel Yvonne Arantes Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract This experimental controlled study was performed to evaluate the composition of autologous processed plasma (APP), and the effects of APP intra-articular injection into healthy equine metacarpophalangeal joints. the effects on joints were analysed with a short-phase protocol and a prolonged-phase protocol using saline-injected joints as controls. for the short protocol, horses received one intra-articular APP injection. Synovial fluid samples were collected prior to the injection and 3, 6, 24, 48, and 16 h after treatment. for the prolonged protocol, the joints received three weekly injections of APP, and samples were collected at 0, 7, 14, 21, and 28 days before APP administration. IL1-ra level was found to be increased in APP compared to plasma. Upon intra-articular administration of APP, transient (up to 24 h) increases in white blood cell (WBC) counts along with elevated protein and prostaglandin E-2 (PGE(2)) concentrations were observed in the treated joints. Over the 28-day observation period, APP did not elicit changes relative to baseline levels, but WBC counts, PGE(2) and chondroitin sulphate concentrations were lower than those found in the control. in conclusion, APP intra-articular injection induced a mild and transitory inflammatory response but no inflammation reaction was observed over a longer period of treatment and observation.
Keywords autologous processed plasma
equine
inflammatory markers
joint
synovial fluid
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2015-03-01
Published in Journal of Veterinary Science. Seoul: Korean Soc Veterinary Science, v. 16, n. 1, p. 47-55, 2015.
ISSN 1229-845X (Sherpa/Romeo, impact factor)
Publisher Korean Soc Veterinary Science
Extent 47-55
Origin http://dx.doi.org/10.4142/jvs.2015.16.1.47
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000351480900007
URI http://repositorio.unifesp.br/handle/11600/38803

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