Cryptic and Rare Aspergillus Species in Brazil: Prevalence in Clinical Samples and in Vitro Susceptibility to Triazoles

Cryptic and Rare Aspergillus Species in Brazil: Prevalence in Clinical Samples and in Vitro Susceptibility to Triazoles

Author Negri, C. E. Autor UNIFESP Google Scholar
Goncalves, S. S. Autor UNIFESP Google Scholar
Xafranski, Hemilio Autor UNIFESP Google Scholar
Bergamasco, M. D. Autor UNIFESP Google Scholar
Aquino, V. R. Google Scholar
Castro, P. T. O. Google Scholar
Colombo, A. L. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
Hosp Canc Barretos
Abstract Aspergillus spp. are among the most common causes of opportunistic invasive fungal infections in tertiary care hospitals. Little is known about the prevalence and in vitro susceptibility of Aspergillus species in Latin America, because there are few medical centers able to perform accurate identification at the species level. the purpose of this study was to analyze the distribution of cryptic and rare Aspergillus species among clinical samples from 133 patients with suspected aspergillosis admitted in 12 medical centers in Brazil and to analyze the in vitro activity of different antifungal drugs. the identification of Aspergillus species was performed based on a polyphasic approach, as well as sequencing analysis of the internal transcribed spacer (ITS) region, calmodulin, and beta-tubulin genes and phylogenetic analysis when necessary. the in vitro susceptibility tests with voriconazole, posaconazole, and itraconazole were performed according to the CLSI M38-A2 document (2008). We demonstrated a high prevalence of cryptic species causing human infection. Only three isolates, representing the species Aspergillus thermomutatus, A. ochraceus, and A. calidoustus, showed less in vitro susceptibility to at least one of the triazoles tested. Accurate identifications of Aspergillus at the species level and with in vitro susceptibility tests are important because some species may present unique resistance patterns against specific antifungal drugs.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2012/01134-8
FAPESP: 2012/01548-7
FAPESP: 2012/19103-1
CAPES: PNPD 23038.007393/2011-11
Date 2014-10-01
Published in Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 52, n. 10, p. 3633-3640, 2014.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 3633-3640
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000342371700016

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