Cryptic and Rare Aspergillus Species in Brazil: Prevalence in Clinical Samples and in Vitro Susceptibility to Triazoles

Cryptic and Rare Aspergillus Species in Brazil: Prevalence in Clinical Samples and in Vitro Susceptibility to Triazoles

Autor Negri, C. E. Autor UNIFESP Google Scholar
Goncalves, S. S. Autor UNIFESP Google Scholar
Xafranski, H. Autor UNIFESP Google Scholar
Bergamasco, M. D. Autor UNIFESP Google Scholar
Aquino, V. R. Google Scholar
Castro, P. T. O. Google Scholar
Colombo, A. L. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
Hosp Canc Barretos
Resumo Aspergillus spp. are among the most common causes of opportunistic invasive fungal infections in tertiary care hospitals. Little is known about the prevalence and in vitro susceptibility of Aspergillus species in Latin America, because there are few medical centers able to perform accurate identification at the species level. the purpose of this study was to analyze the distribution of cryptic and rare Aspergillus species among clinical samples from 133 patients with suspected aspergillosis admitted in 12 medical centers in Brazil and to analyze the in vitro activity of different antifungal drugs. the identification of Aspergillus species was performed based on a polyphasic approach, as well as sequencing analysis of the internal transcribed spacer (ITS) region, calmodulin, and beta-tubulin genes and phylogenetic analysis when necessary. the in vitro susceptibility tests with voriconazole, posaconazole, and itraconazole were performed according to the CLSI M38-A2 document (2008). We demonstrated a high prevalence of cryptic species causing human infection. Only three isolates, representing the species Aspergillus thermomutatus, A. ochraceus, and A. calidoustus, showed less in vitro susceptibility to at least one of the triazoles tested. Accurate identifications of Aspergillus at the species level and with in vitro susceptibility tests are important because some species may present unique resistance patterns against specific antifungal drugs.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Número do financiamento FAPESP: 2012/01134-8
FAPESP: 2012/01548-7
FAPESP: 2012/19103-1
CAPES: PNPD 23038.007393/2011-11
Data 2014-10-01
Publicado em Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 52, n. 10, p. 3633-3640, 2014.
ISSN 0095-1137 (Sherpa/Romeo, fator de impacto)
Editor Amer Soc Microbiology
Extensão 3633-3640
Fonte http://dx.doi.org/10.1128/JCM.01582-14
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000342371700016
URI http://repositorio.unifesp.br/handle/11600/38240

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