The effect of NaCl on the adsorption of human IgG onto CM-Asp-PEVA hollow fiber membrane-immobilized nickel and cobalt metal ions

The effect of NaCl on the adsorption of human IgG onto CM-Asp-PEVA hollow fiber membrane-immobilized nickel and cobalt metal ions

Author Pavan, Gisele Luiza Google Scholar
Lazzarotto Bresolin, Igor Tadeu Autor UNIFESP Google Scholar
Borsoi-Ribeiro, Mariana Google Scholar
Vijayalakshmi, Mookambesvaran Google Scholar
Alves Bueno, Sonia Maria Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
VIT Univ
Abstract Over the past decade, immobilized metal-affinity adsorbents have attracted increasing interest for purification of natural and recombinant immunoglobulin G (IgG). in this work, nickel and cobalt metal ions complexed with CM-Asp (carboxymethylaspartate) immobilized on poly(ethylenevinyl alcohol) (PEVA) hollow fiber membranes were evaluated for purification of human IgG from serum. the buffer system and NaCl had important effects on human serum protein adsorption in both adsorbents. Efficient purification of IgG was accomplished in sodium phosphate buffer without NaCl at pH 7.0. Under this condition, the electrostatic interactions are important for adsorption. the Ni(II)-CM-Asp-PEVA had a protein adsorption capacity of 17.5 mg of IgG mL(-1) fiber when human serum diluted was loaded in crossflow filtration mode and the eluted IgG had a purity of 82.6 % (based on total protein and IgG, IgM, HSA, and Trf nephelometric analysis). Fitting the experimental IgG adsorption data to the Langmuir and Langmuir-Freundlich models showed that Ni(II)-CM-Asp and Co(II)-CM-Asp had Langmuirean and non-Langmuirean behavior, respectively, with positive cooperativity for IgG-Co(II)-CM-Asp binding, probably due to multipoint interactions (n = 2.12 +/- A 0.31). Thus, these membranes can be considered as alternative adsorbents for the purification or depletion of IgG from human serum.
Keywords Human IgG
Purification
Affinity membrane
IMAC
CM-Asp
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-08-01
Published in Adsorption-Journal of the International Adsorption Society. Dordrecht: Springer, v. 20, n. 5-6, p. 677-688, 2014.
ISSN 0929-5607 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 677-688
Origin http://dx.doi.org/10.1007/s10450-014-9612-1
Access rights Closed access
Type Article
Web of Science ID WOS:000339103300003
URI http://repositorio.unifesp.br/handle/11600/38032

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