c-FOS Expression After Hippocampal Deep Brain Stimulation in Normal Rats

c-FOS Expression After Hippocampal Deep Brain Stimulation in Normal Rats

Author Silva, Jose C. da Autor UNIFESP Google Scholar
Scorza, Fulvio A. Autor UNIFESP Google Scholar
Nejm, Mariana B. Autor UNIFESP Google Scholar
Cavalheiro, Esper A. Autor UNIFESP Google Scholar
Cukiert, Arthur Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract ObjectivesWe studied the effects of Hip-deep brain stimulation (DBS) on the expression of the inducible transcription factor c-FOS in the brain of normal rats.Materials and MethodsTen Wistar rats were anesthetized, and nine were implanted with epidural and hippocampal electrodes for brain activity recording; one animal was used as sham. Bipolar stimulating electrodes were implanted in the left hippocampus. Three animals were used as control (implanted but not stimulated), one as sham (not implanted, not stimulated), and six as the study group. Stimulation was carried out using square wave pulses with 0.8V, 300 mu sec, and 130 Hz (similar to 25 mu C/cm2) on the left hippocampus through the implanted bipolar hippocampal lead. Three animals were submitted to a one-hour and three to a six-hour stimulation session. Immunohistochemistry was employed to visualize c-FOS distribution in the rat's brain. the presence of seizures and electrocorticographic findings also were observed.ResultsIn animals submitted to both one-hour or six-hour unilateral hippocampal stimulation sessions, there was a significant bilateral overexpression of c-FOS in the hippocampus proper, dentate gyrus, and hylus. in the CA1 and CA3 regions, although activation was bilateral, c-FOS hyperexpression prevailed at the stimulated side over time; this was not true for the hilar and dentate gyrus regions where a more symmetric activation occurred over time. A significant c-FOS activation occurred after one hour of Hip-DBS in the ipsilateral amygdala; there was no contralateral amygdala activation, and by six hours, no amygdala activation was noted. No c-FOS activation was noted in other brain areas.DiscussionOur data showed that unilateral Hip-DBS was able to cause widespread and persistent bilateral activation of the normal rat limbic system, although in some, nuclei activation prevailed over the stimulated side. Cortical activation outside the limbic system was not noted. Our data represent a first approach to study the mechanistic paradigm involved in Hip-DBS.
Keywords hippocampal stimulation
rat
c-FOS expression
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-04-01
Published in Neuromodulation. Hoboken: Wiley-Blackwell, v. 17, n. 3, p. 213-217, 2014.
ISSN 1094-7159 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 213-217
Origin http://dx.doi.org/10.1111/ner.12122
Access rights Closed access
Type Article
Web of Science ID WOS:000335154500004
URI http://repositorio.unifesp.br/handle/11600/37644

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