Reproductive Function of the Male Obese Zucker Rats: Alteration in Sperm Production and Sperm DNA Damage

Reproductive Function of the Male Obese Zucker Rats: Alteration in Sperm Production and Sperm DNA Damage

Author Vendramini, V. Autor UNIFESP Google Scholar
Cedenho, A. P. Autor UNIFESP Google Scholar
Miraglia, S. M. Autor UNIFESP Google Scholar
Spaine, D. M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Obesity has been considered a public health issue in many countries and is of increasing concern for authorities over the past 6 years. the Zucker rat is a good experimental model for obesity and diabetes studies due to its metabolic characteristics that are similar to those developed by humans. A total of 12 obese Zucker rats and their lean littermates were killed in pubertal and young adult phases for assessing organ weights (testis and epididymis), testicular histomorphometric and stereological analyses, daily sperm production, and transit time in the epididymis. Sperm integrity was also investigated in the adult animals using the Comet assay. Alterations in organ weights, seminiferous epithelium architecture, sperm production, and transit time were noticed in the pubertal fatty rats. the volume density of the lymphatic space was decreased in both the ages. Adult animals had a significant increase in the extent of damage found in sperm DNA. Our results show for the first time that leptin receptor deficiency compromises sperm production during puberty and that genetic obese Zucker rats have increased sperm DNA fragmentation.
Keywords Zucker rat
obesity
spermatogenesis
sperm production
DNA fragmentation
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Division of Urology
Discipline of Developmental Biology laboratory at UNIFESP
Date 2014-02-01
Published in Reproductive Sciences. Thousand Oaks: Sage Publications Inc, v. 21, n. 2, p. 221-229, 2014.
ISSN 1933-7191 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Inc
Extent 221-229
Origin http://dx.doi.org/10.1177/1933719113493511
Access rights Closed access
Type Article
Web of Science ID WOS:000329197700009
URI http://repositorio.unifesp.br/handle/11600/37417

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