Establishment and Partial Characterization of an Epirubicin-Resistant Gastric Cancer Cell Line with Upregulated ABCB1

Establishment and Partial Characterization of an Epirubicin-Resistant Gastric Cancer Cell Line with Upregulated ABCB1

Author Felipe, Aledson Vitor Autor UNIFESP Google Scholar
Moraes, Andrea Aparecida Autor UNIFESP Google Scholar
Oliveira, Juliana de Autor UNIFESP Google Scholar
Silva, Tiago Donizetti da Autor UNIFESP Google Scholar
Forones, Nora Manoukian Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Nove de Julho
Abstract Multidrug resistance (MDR) is a major impediment to successful chemotherapy of gastric cancer. Our aim was to establish an epirubicin-resistant cell subline (AGS/EPI) and to elucidate the mechanisms involved in acquired EPI resistance. the AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. An AGS/EPI cell subline could thus be effectively established, and MDR mechanism of these cells was shown to be related to the overexpression of mRNA of the ABCB1 gene.
Keywords Multidrug resistance
gastric cancer
ABCB1
epirubicin
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-01-01
Published in Asian Pacific Journal of Cancer Prevention. Gyeonggi-do: Asian Pacific Organization Cancer Prevention, v. 15, n. 16, p. 6849-6853, 2014.
ISSN 1513-7368 (Sherpa/Romeo, impact factor)
Publisher Asian Pacific Organization Cancer Prevention
Extent 6849-6853
Origin http://dx.doi.org/10.7314/APJCP.2014.15.16.6849
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000343832900065
URI http://repositorio.unifesp.br/handle/11600/37203

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