Macrophage Trafficking as Key Mediator of Adenine-Induced Kidney Injury

Macrophage Trafficking as Key Mediator of Adenine-Induced Kidney Injury

Autor Correa-Costa, Matheus Google Scholar
Braga, Tarcio Teodoro Google Scholar
Felizardo, Raphael Jose Ferreira Autor UNIFESP Google Scholar
Andrade-Oliveira, Vinicius Google Scholar
Regina Perez, Katia Autor UNIFESP Google Scholar
Midea Cuccovia, Iolanda Google Scholar
Ioshie Hiyane, Meire Google Scholar
Santana da Silva, Joao Google Scholar
Saraiva Camara, Niels Olsen Autor UNIFESP Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Macrophages play a special role in the onset of several diseases, including acute and chronic kidney injuries. in this sense, tubule interstitial nephritis (TIN) represents an underestimated insult, which can be triggered by different stimuli and, in the absence of a proper regulation, can lead to fibrosis deposition. Based on this perception, we evaluated the participation of macrophage recruitment in the development of TIN. Initially, we provided adenine-enriched food to WT and searched for macrophage presence and action in the kidney. Also, a group of animals were depleted of macrophages with the clodronate liposome while receiving adenine-enriched diet. We collected blood and renal tissue from these animals and renal function, inflammation, and fibrosis were evaluated. We observed higher expression of chemokines in the kidneys of adenine-fed mice and a substantial protection when macrophages were depleted. Then, we specifically investigated the role of some key chemokines, CCR5 and CCL3, in this TIN experimental model. Interestingly, CCR5 KO and CCL3 KO animals showed less renal dysfunction and a decreased proinflammatory profile. Furthermore, in those animals, there was less profibrotic signaling. in conclusion, we can suggest that macrophage infiltration is important for the onset of renal injury in the adenine-induced TIN.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Instituto Nacional de Ciencia e Tecnologia de Fluidos Complexos (INCT Complex Fluids)
Número do financiamento FAPESP: 2009/54474-8
FAPESP: 2012/02270-2
FAPESP: 2013/25010-9
Data de publicação 2014-01-01
Publicado em Mediators of Inflammation. New York: Hindawi Publishing Corporation, 12 p., 2014.
ISSN 0962-9351 (Sherpa/Romeo, fator de impacto)
Publicador Hindawi Publishing Corporation
Extensão 12
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000339770900001
Endereço permanente

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