Optical neuritis induced by different concentrations of myelin oligodendrocyte glycoprotein presents different profiles of the inflammatory process

Optical neuritis induced by different concentrations of myelin oligodendrocyte glycoprotein presents different profiles of the inflammatory process

Author Gibaile Soares, Rubens Murilo Google Scholar
Dias, Alyria Teixeira Google Scholar
Ribeiro de Castro, Sandra Bertelli Google Scholar
Souza Alves, Caio Cesar Google Scholar
Evangelista, Marcilene Gomes Google Scholar
Da Silva, Luan Cristian Google Scholar
Farias, Rogerio Estevam Google Scholar
Marques Nogueira Castanon, Maria Christina Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Ferreira, Ana Paula Google Scholar
Institution Univ Fed Juiz de Fora
Universidade Federal de São Paulo (UNIFESP)
Abstract Optical neuritis (ON) is characterized by inflammation of the optic nerve, and is one of the first clinical signs of multiple sclerosis (MS). Experimental autoimmune encephalomyelitis (EAE) is the animal model used to study MS and ON. the present study evaluated the induction, development and progression of ON using an EAE model induced by 100 mu g or 300 mu g of MOG(35-55). An EAE model was induced in C57BL/6 mice by tail base injection of 100 mu g or 300 mu g of MOG(35-55) in complete Freund's adjuvant, supplemented with Mycobacterium tuberculosis. On the day of injection and 48 h later, animals received intraperitoneally 300 ng of pertussis toxin. On days 7, 10, 14, 21 and 58 the optic nerve was dissected for histological analysis, production of CCL5 and immunohistochemical detection of CD4 and CD8. the histological changes observed in the optic nerves consisted of inflammatory cell infiltrates showing varying degrees of ON in the two groups. the onset of ON in the 300 mu g of MOG(35-55) group was coincident with higher production of CCL5, on day 10 after induction. However, the 100 mu g MOG(35-55) group showed more intense inflammatory infiltrate on day 14 after induction, with higher amounts of CD4 and CD8, reaching an excessive demyelination process on days 21 and 58 after induction. the results suggest that two different concentrations of MOG(35-55) lead to different forms of evolution of optic neuritis.
Keywords Experimental autoimmune encephalomyelitis
immunohistochemistry
multiple sclerosis
MOG
optic nerve
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CNPq: 481459/2009-0
CNPq: 303369/2009-4
CNPq: 150017/2013-8
FAPEMIG: 02236/10
FAPEMIG: PPM 0216/10
CAPES: PNPD-2882/2011
Date 2013-11-01
Published in Autoimmunity. London: Informa Healthcare, v. 46, n. 7, p. 480-485, 2013.
ISSN 0891-6934 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 480-485
Origin http://dx.doi.org/10.3109/08916934.2013.796938
Access rights Closed access
Type Article
Web of Science ID WOS:000325276200009
URI http://repositorio.unifesp.br/handle/11600/36967

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