Methylation Pattern of THBS1, GATA-4, and HIC1 in Pediatric and Adult Patients Infected with Helicobacter pylori

Methylation Pattern of THBS1, GATA-4, and HIC1 in Pediatric and Adult Patients Infected with Helicobacter pylori

Author Alvarez, Marisa Claudia Google Scholar
Placido Ladeira, Marcelo Sady Google Scholar
Affonso Scaletsky, Isabel Cristina Autor UNIFESP Google Scholar
Pedrazzoli, Jose Google Scholar
Ribeiro, Marcelo Lima Google Scholar
Institution Univ Sao Francisco
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background Helicobacter pylori infection is usually acquired in childhood and persists into adulthood if untreated. the bacterium induces a chronic inflammatory response, which is associated with epigenetic alterations in oncogenes, tumor-suppressor genes, cell-cycle regulators, and cell-adhesion molecules.Aim the aim of this study was to analyze the effect of H. pylori infection on the methylation status of Thrombospondin-1 (THBS1), Hypermethylated in cancer 1 (HIC1) and Gata binding protein-4 (GATA-4) in gastric biopsy samples from children and adults infected or uninfected with the bacterium and in samples obtained from gastric cancer patients.Methods the methylation pattern was analyzed with methylation-specific PCR.Results Our results showed that H. pylori infection was associated with methylation of the promoter regions of the THBS1 and GATA-4 genes in pediatric and adult samples (p < 0.01). HIC1 showed the lowest level of methylation, which was not an early event during gastric carcinogenesis.Conclusions the results from this study indicate that methylation of THBS1 and GATA-4 occurs in the early stages of chronic gastritis and gastric cancer in association with H. pylori infection; however, in gastric cancer samples, other mechanisms cooperate with the down-regulation of these genes. Methylation of HIC1 may not be the principal mechanism implicated in its down-regulation in gastric cancer samples.
Keywords Chronic gastritis
Gastric cancer
Methylation
Helicobacter pylori
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2008/02678-6
Date 2013-10-01
Published in Digestive Diseases and Sciences. Dordrecht: Springer, v. 58, n. 10, p. 2850-2857, 2013.
ISSN 0163-2116 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 2850-2857
Origin http://dx.doi.org/10.1007/s10620-013-2742-6
Access rights Closed access
Type Article
Web of Science ID WOS:000324778900024
URI http://repositorio.unifesp.br/handle/11600/36799

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